M0888

Manufactured by Agilent Technologies

Sourced in United States, Germany

The M0888 is a general-purpose laboratory equipment used for various analytical and experimental purposes. It is designed to perform specific functions within a laboratory setting. The core functionality of the M0888 is to provide a controlled and reliable environment for conducting experiments or analyses. Due to the technical nature of the product, a more detailed description without interpretation or extrapolation cannot be provided.

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Lab products found in correlation

Anti β catenin, by Merck Group (1 mentions) Cell conditioner 1, by Roche (1 mentions) Hematoxylin and bluing reagent, by Roche (1 mentions) E cadherin, by Roche (1 mentions) Opti view hq linker, by Roche (1 mentions) Benchmark ultra, by Roche (1 mentions) A8273, by Merck Group (1 mentions) Rabbit anti β actin, by Cell Signaling Technology (1 mentions) Ab30788, by Abcam (1 mentions) Fluorescent secondary antibody, by Jackson ImmunoResearch (1 mentions) Ab6308, by Abcam (1 mentions) G2654, by Merck Group (1 mentions) Mab1926, by Merck Group (1 mentions) Autostainer, by Leica (1 mentions) M7103, by Agilent Technologies (1 mentions) Ncl l cd4 1f6, by Leica (1 mentions) M7158, by Agilent Technologies (1 mentions) M7050, by Agilent Technologies (1 mentions) M7240, by Agilent Technologies (1 mentions) M0851, by Agilent Technologies (1 mentions)

6 protocols using m0888

Immunohistochemistry Staining Protocol

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Sections were pretreated in CC1-buffer (Cell Conditioner 1; Ventana Medical Systems, Inc., Tucson, AZ, USA) at 95°C for 36 min (E-cadherin, β-catenin), at 95°C for 64 min (CK19) and at 100°C for 36 min (CK5). Slides were then incubated with primary antibodies diluted in Ventana antibody diluent for 32 min at 36°C and detected using Ultra View Universal DAB Detection kit using a Bench Mark Ultra (Ventana Medical Systems, Inc.). For slides stained with CK5 an extra step adding an Opti View HQ Linker (Ventana Medical Systems, Inc.) was added before detection. Slides were counterstained with Hematoxylin and Bluing Reagent (Ventana Medical Systems, Inc.). The antibody against E-cadherin (M3612, DAKO; Agilent Technologies, Inc., Santa Clara, CA, USA) was diluted 1:25, anti-β-catenin (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) 1:1,500, anti-CK-5 (Novocastra; Leica Microsystems, Inc., Buffalo Grove, IL, USA) 1:100 and anti-CK-19 (M0888, DAKO; Agilent Technologies, Inc.) 1:50.

Sgaramella N., Wilms T., Boldrup L., Loljung L., Gu X., Coates P.J., Hassellöf P., Califano L., Lo Muzio L., Fåhraeus R., Norberg Spaak L., Franco R., Tartaro G., Colella G., Santagata M., Dell'Aversana Orabona G., Chirico F., Danielsson K., Troiano G., Ardito F, & Nylander K. (2018). Ethnicity based variation in expression of E-cadherin in patients with squamous cell carcinoma of the oral tongue. Oncology Letters, 16(5), 6603-6607.

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Protein Expression Analysis of Exocrine Cells

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Freshly isolated or cultured exocrine cells (approximately 10⁶ cells) were harvested and washed with PBS, and subsequently lysed with 250 μl of radio immunoprecipitation assay (RIPA) buffer supplemented with 1/10 β-mercaptoethanol. The protein concentration was determined by the Bradford method and 50 μg was used for SDS gel electrophoresis. Upon blotting on to nitrocellulose membranes, samples were incubated overnight at 4°C with primary antibodies dilutions (rabbit anti-β-actin, 1/2000, 4967S, Cell Signalling Technology, Bioke; rabbit anti-Amylase, 1/1000, A8273, Sigma–Aldrich; mouse anti-CK19, 1/1000, M0888, Dako). Membranes were washed and exposed to the secondary antibodies (goat anti-rabbit, 1/1000, Sigma–Aldrich or goat anti-mouse, 1/1000, Santa Cruz Biotechnologies) at room temperature for 1h, and the detection was performed using the chemiluminescence method.

Mfopou J.K., Houbracken I., Wauters E., Mathijs I., Song I., Himpe E., Baldan J., Heimberg H, & Bouwens L. (2016). Acinar phenotype is preserved in human exocrine pancreas cells cultured at low temperature: implications for lineage-tracing of β-cell neogenesis. Bioscience Reports, 36(3), e00329.

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Immunohistochemical Characterization of Fetal Pancreas

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Human fetal pancreas samples were fixed in 4% paraformaldehyde and paraffin embedded. Sections (4 μm) were prepared and stained with either appropriate dilutions of primary antibodies, including CK19 (M0888, Dako), pancreatic and duodenal homeobox 1 (PDX-1; Dr. Wright, University of Vanderbilt), insulin (18-0067, Invitrogen, Camarillo, CA, USA), glucagon (G2654, Sigma), somatostatin (ab30788, Abcam), pancreatic polypeptide (18-0043, Zymed), amylase (171534, Calbiochem), collagen I (ab6308, Abcam), collagen IV (MAB1910), laminin (AB19012) and fibronectin (MAB1926, Millipore) or Schiff's reagent for PAS staining (24200-1, Polysciences Inc.).¹⁶ (link) Slides were then treated with either fluorescent secondary antibodies (obtained from Jackson Immunoresearch Laboratories) or haematoxylin as a counterstain, and at least 3 pancreata per age group were examined.

Riopel M., Li J., Fellows G.F., Goodyer C.G, & Wang R. (2014). Ultrastructural and immunohistochemical analysis of the 8-20 week human fetal pancreas. Islets, 6(4), e982949.

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Autopsy Examination Protocol

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An autopsy was performed two hours after the patient’s death. No craniotomy was performed. Written informed consent was obtained from the patient’s family before autopsy. The protocol for reporting this case was approved by the ethics committee of Asahikawa Medical University (approval number #17115, August 28, 2017). The systemic organs were removed, examined, and fixed in phosphate-buffered 10 % formalin. Tissues were processed to paraffin blocks and the Section (4 μm) were subjected to hematoxylin and eosin staining, as well as Berlin blue staining. An autostainer (Leica Biosystems, Nußloch, Germany) was used to perform immunohistochemistry assessments of CD79a (antibody: M7050, DAKO, Carpinteria, CA), CD4 (antibody: NCL-L-CD4-1F6, Novocastra, Leica Microsystems, Wetzlar, Germany), CD8 (antibody: M7103, DAKO), HepPar1 (antibody: M7158, DAKO), cytokeratin 19 (CK19) (antibody: M0888, DAKO), and Ki-67 (MIB-1) (M7240, DAKO) expression.

Nishikawa Y., Matsuo Y., Watanabe R., Miyazato M., Matsuo M., Nagahama Y., Tanaka H., Ooshio T., Goto M., Okada Y, & Fujita S. (2023). Hepatocyte-specific damage in acute toxicity of sodium ferrous citrate: Presentation of a human autopsy case and experimental results in mice. Toxicology Reports, 10, 669-679.

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Immunofluorescence Staining of Tissue Samples

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Slides from frozen tissue samples or cultured cells were available for IF. Staining was performed using the following antibodies: αSMA mouse monoclonal (1 : 2, M-0851 (Dako, Les Ulis, France) or 1 : 200, A2547 (Sigma-Aldrich)), SLIT2 rabbit polyclonal (1 : 40, sc-28945; Santa Cruz Biotechnology, Heidelberg, Germany) and cytokeratin 19 mouse monoclonal (1 : 50, M-0888, Dako). Image quantification was carried out using Image J software (http://imagej.nih.gov/ij/).

Secq V., Leca J., Bressy C., Guillaumond F., Skrobuk P., Nigri J., Lac S., Lavaut M.N., Bui T.T., Thakur A.K., Callizot N., Steinschneider R., Berthezene P., Dusetti N., Ouaissi M., Moutardier V., Calvo E., Bousquet C., Garcia S., Bidaut G., Vasseur S., Iovanna J.L, & Tomasini R. (2015). Stromal SLIT2 impacts on pancreatic cancer-associated neural remodeling. Cell Death & Disease, 6(1), e1592-.

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Immunohistochemical Staining for Cell Markers

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The immunohistochemical stain for CK7 (1 : 200, Dako M7018, mouse monoclonal, clone OV-TL 12/20), CK19 (1 : 50, Dako M0888, mouse monoclonal, clone RCK108), and CD34 (1 : 160, Dako M7165, mouse monoclonal, clone QBEnd 10) was performed on the 5-micrometer thick tissue sections of paraffin-embedded tissue blocks. Antigen retrieval was carried out with 0.01 M citrate buffer at pH 6.0. The slides were stained on the Dako Autostainer (Dako Corporation, Carpinteria, CA).
Sections of various control tissues (breast for CK7, liver for CK19, and colon for CD34) with known positivity for the target proteins were used as positive stain controls. Positive staining was defined as dark brown staining pattern. Scant or fine granular background staining or no staining at all was considered negative.

Lee H., Ainechi S., Dresser K, & Kurian E.M. (2014). Central Portalization Correlates with Fibrosis but Not with Risk Factors for Nonalcoholic Steatohepatitis in Steatotic Chronic Hepatitis C. International Journal of Hepatology, 2014, 329297.

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