After culturing overnight, 10 μL of S. pyogenes was inoculated onto the surface of a hyaluronan agar plate containing Todd-Hewitt broth (BD Biosciences), 1% yeast extract (BD Biosciences), 400 μg/mL hyaluronic acid sodium salt (Wako), 1% bovine serum albumin (Sigma), and 2% Noble agar (BD Difco, Franklin Lakes, NJ, USA). Following overnight incubation, agar plates were flooded with 2 N acetic acid solution and further incubated for 15 minutes. Strains exhibiting a halo zone around the colony were deemed positive for hyaluronidase activity. S. pyogenes strain 5448 (serotype M1, negative for hyaluronidase activity) and strain 4063-05 (serotype M4, positive for hyaluronidase activity) were employed as positive and negative controls, respectively.20 (link)
Hyaluronic acid sodium salt
Manufactured by Fujifilm
Sourced in United States
Hyaluronic acid sodium salt is a key component in various laboratory applications. It is a naturally occurring polysaccharide that functions as a lubricant and shock absorber in joints and other tissues. This product can be used in applications where the unique properties of hyaluronic acid are required.
Automatically generated - may contain errors
Lab products found in correlation
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Yeast extract, by BD (1 mentions)
Todd hewitt broth, by BD (1 mentions)
Polyvinylpyrrolidone pvp, by Merck Group (1 mentions)
Poly l lysine hydrobromide pll, by Merck Group (1 mentions)
Poly ethylene glycol, by Nacalai Tesque (1 mentions)
Poly l ornithine hydrobromide plo, by Merck Group (1 mentions)
Chondroitin sulfate a, by Merck Group (1 mentions)
Poly d lysine hydrobromide, by Merck Group (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
2 protocols using hyaluronic acid sodium salt
1
Assay for Streptococcal Hyaluronidase Activity
2
Polymer-Supplemented Cell Culture Media
Check if the same lab product or an alternative is used in the 5 most similar protocols
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High-molecular-weight (M.W.) soluble polymers and their final concentration in culture
medium (CiCM) were as follows: Poly-ethylene glycol (PEG) [M.W. 15000-25000, nacalai
tesque]: CiCM 0.5-1 mg/ml; polyvinyl alcohol (PVA) [M.W. 146000-186000, Sigma-Aldrich]:
CiCM 0.5-1 mg/ml; polyvinylpyrrolidone (PVP) [M.W. 360000, Sigma-Aldrich]: CiCM 1 mg/ml;
dextran (Dex) [M.W. 190000-230000, nacalai tesque]: CiCM 1 mg/ml; poly-γ-glutamic acid
(PGA) [M.W. 200000-500000, Wako Pure Chemical industry]: CiCM 1 mg/ml; chondroitin sulfate
C sodium salt (CSC) [M.W. 40000-80000,Wako Pure Chemical industry]: CiCM 0.5 mg/ml;
hyaluronic acid sodium salt (HRL) [M.W. not determined, Wako Pure Chemical industry]: CiCM
0.5 mg/ml; chondroitin sulfate A sodium salt (CSA) [M.W. 40000-80000, Sigma-Aldrich]: CiCM
0.5 mg/ml; dermatan sulfate sodium salt (DS) [M.W. 85000~100000, Tokyo Chemical Industry]:
CiCM 0.5 mg/ml; poly-L-lysine hydrobromide (PLL) [M.W. 30000-70000, Sigma-Aldrich] CiCM
5-50 μg/ml; poly-L-ornithine hydrobromide (PLO) [M.W. 30000-70000, Sigma-Aldrich]: CiCM
12.5 μg/ml; poly-D-lysine hydrobromide (PDL) [M.W. 30000-70000, Sigma-Aldrich]: CiCM 12.5
μg/ml; poly-L-histidine (PLH) [M.W. 5000-25000, Sigma-Aldrich]: CiCM 12.5 μg/ml.
medium (CiCM) were as follows: Poly-ethylene glycol (PEG) [M.W. 15000-25000, nacalai
tesque]: CiCM 0.5-1 mg/ml; polyvinyl alcohol (PVA) [M.W. 146000-186000, Sigma-Aldrich]:
CiCM 0.5-1 mg/ml; polyvinylpyrrolidone (PVP) [M.W. 360000, Sigma-Aldrich]: CiCM 1 mg/ml;
dextran (Dex) [M.W. 190000-230000, nacalai tesque]: CiCM 1 mg/ml; poly-γ-glutamic acid
(PGA) [M.W. 200000-500000, Wako Pure Chemical industry]: CiCM 1 mg/ml; chondroitin sulfate
C sodium salt (CSC) [M.W. 40000-80000,Wako Pure Chemical industry]: CiCM 0.5 mg/ml;
hyaluronic acid sodium salt (HRL) [M.W. not determined, Wako Pure Chemical industry]: CiCM
0.5 mg/ml; chondroitin sulfate A sodium salt (CSA) [M.W. 40000-80000, Sigma-Aldrich]: CiCM
0.5 mg/ml; dermatan sulfate sodium salt (DS) [M.W. 85000~100000, Tokyo Chemical Industry]:
CiCM 0.5 mg/ml; poly-L-lysine hydrobromide (PLL) [M.W. 30000-70000, Sigma-Aldrich] CiCM
5-50 μg/ml; poly-L-ornithine hydrobromide (PLO) [M.W. 30000-70000, Sigma-Aldrich]: CiCM
12.5 μg/ml; poly-D-lysine hydrobromide (PDL) [M.W. 30000-70000, Sigma-Aldrich]: CiCM 12.5
μg/ml; poly-L-histidine (PLH) [M.W. 5000-25000, Sigma-Aldrich]: CiCM 12.5 μg/ml.
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