Zinc acetate dihydrate was purchased from Sigma Aldrich (St. Louis, MO, USA); magnesium acetate tetrahydrate was purchased from Fisher Scientific (Waltham, MA, USA); sodium hydroxide was purchased from Sigma Aldrich; and 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB) was purchased from Thermo Fisher Scientific (Heysham, UK). MCF-7 cells, Dulbecco’s Modified Eagle’s Medium (DMEM), penicillin/streptomycin, and fetal bovine serum (FBS) were purchased from ATCC (Manassas, VA, USA). The CyQuant™ MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) Cell Viability Assay was purchased from Thermo Fisher Scientific (Waltham, MA, USA).
5 5 dithiobis 2 nitrobenzoic acid dtnb
Manufactured by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Belgium
5,5′-dithiobis (2-nitrobenzoic acid) (DTNB) is a chemical compound that is commonly used as a reagent in biochemical and analytical applications. It is a yellow, crystalline solid that is soluble in water and organic solvents. DTNB is used to detect and quantify the presence of thiol groups (SH) in proteins and other biomolecules.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Merck Group (3 mentions)
Potassium phosphate monobasic, by Thermo Fisher Scientific (3 mentions)
Methanol, by Merck Group (3 mentions)
Sodium carbonate, by Merck Group (2 mentions)
Potassium chloride (kcl), by Merck Group (2 mentions)
Sucrose, by Merck Group (2 mentions)
Potassium phosphate dibasic, by Thermo Fisher Scientific (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
Milli q high purity water unit, by Merck Group (2 mentions)
9 10 phenanthrenequinone pqn, by Thermo Fisher Scientific (2 mentions)
Dl dithiothreitol dtt, by Thermo Fisher Scientific (2 mentions)
Ascorbic acid, by Merck Group (2 mentions)
Chelex 100 sodium form, by Merck Group (2 mentions)
Horseradish peroxidase, by Merck Group (2 mentions)
Zincon sodium salt, by Merck Group (2 mentions)
Zncl2, by Merck Group (2 mentions)
Hepes, by Merck Group (2 mentions)
Iodoacetamide, by Merck Group (2 mentions)
Mcf 7 cell, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by American Type Culture Collection (1 mentions)
15 protocols using 5 5 dithiobis 2 nitrobenzoic acid dtnb
1
Cytotoxicity Assay for MCF-7 Cells
2
Acetylcholinesterase Inhibition Assay
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Bovine serum albumin (BSA) was purchased from VWR International (Radnor, PA), while acetylthiocholine iodide (ATCI), sodium citrate, sodium chloride, potassium chloride, sodium carbonate, monosodium phosphate, and disodium phosphate were purchased from Sigma‐Aldrich (St. Louis, MO). 2‐[4‐(2‐hydroxyethyl)piperazin‐1‐yl]ethanesulfonic acid (HEPES) was purchased from Hampton Research. Hexanes, 5,5′‐dithiobis(2‐nitrobenzoic acid) (DTNB), and isopropyl alcohol were purchased from Thermo Fisher Scientific (Waltham, MA). Laboratory deionized water of >17 MΩ was used for all assays.
3
Organophosphate Sensing Hydrogel Formulation
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Pyridine-2-aldoxime, bromoacetic acid, chitosan (medium molecular weight), dimethyl formamide, potassium carbonate, O-(benzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HBTU), parathion methyl (PESTANAL), methyl paraoxon (PESTANAL), chlorpyrifos (PESTANAL), carbopol 940, glycerin, propylene glycol, triethanolamine (TEA; S. D. Fine-Chem Limited, Bangalore), snake skin dialysis membrane (MWCO, 3.5 kDa; Thermo Fisher Scientific), trifluoroacetic acid, pNP (Alfa Aesar), isoflurane (Isotroy), Triton X-100, EDTA (Thermo Fisher Scientific), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), acetylthiocholine iodide (ASChI), and deionized water were used. Unless mentioned otherwise, all chemicals were procured from Sigma-Aldrich.
4
Synthesis and Characterization of iTEP-Sali-ABA
5
In Vitro and In Vivo BV Experiments
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For in vitro experiments, BV (Frontier Scientific, Logan, UT, USA) was first dissolved in 0.1% DMSO (Sigma-Aldrich, St Louis, MO, USA) and then adjusted to an appropriate final concentration using prewarmed culture medium as described by Dortay et al (2011) (link). In all in vitro experiments where BV was used, the corresponding amount of DMSO was added to the medium to ensure appropriate control conditions. For in vivo study, BV was dissolved in PBS and neutralised with 1 N HCl to a pH of 7.4, and a final concentration of 1 mM . Subsequently, the solution was sterilised by filtration and stored at −80 °C. All experiments were carried out in a controlled manner to avoid direct light exposure. 5,5′-Dithio-bis-(2-nitrobenzoic acid), (DTNB) was purchased from Thermo Scientific (Rockford, IL, USA). 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) was purchased from Cayman chemical (Ann Arbor, MI, USA). Barium chloride dihydrate and benzene were purchased from Sigma-Aldrich. Matrigel was purchased from BD Biosciences (San Jose, CA, USA).
6
Phytochemical Analysis of Tartary Buckwheat
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TB grains were provided by Bongpyung-nongwon Co. Ltd. (Gangwon-do, Korea). Ethanol, tannic acid, acetic acid, l -ascorbic acid, sodium chloride, sodium acetate, acetonitrile, mEthanol, and sodium phosphate were acquired from Samchun Pure Chemical Co., Ltd. (Pyeongtaek, Korea). Rutin trihydrate was provided by Alfa Aesar (Ward Hill, MA, USA). Sulfatase from Helix pomatia, β-glucuronidase from bovine liver, quercetin, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), ethylene glycol-bis (β-aminoethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA), mannitol, and sucrose were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline (PBS) was provided by Welgene Inc. (Gyeongsan, Korea). Bio-Rad protein assay dye reagent from Bio-Rad Laboratories, Inc. (Hercules, CA, USA), superoxide dismutase (SOD) assay kit from Cayman Chemical Company (Ann Arbor, MI, USA), (+)-catechin, catalase (CAT) assay kit, and glutathione reductase (GR) assay kit from ENZO Life Sciences (Farmingdale, NY, USA), lipid peroxidation (malondialdehyde; MDA) assay kit from abcam (Cambridge, UK), 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNB) from Thermo Fisher Scientific, Inc. (Waltham, MA, USA), and l -cysteine hydrochloride monohydrate from Junsei Chemical Co., Ltd. (Kyoto, Japan) were used.
7
Conjugation of Maleimide-MMAE to HB22.7 Antibody
8
Oxidative Stress Assessment Assay
9
Oxidative Stress Assessment Assay
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Chemicals and gases were obtained from Sigma-Aldrich unless otherwise indicated and were used without further purification: ascorbic acid (≥99.0 %), Chelex™ 100 sodium form, 0.1 M HCl solution, 0.1 M NaOH solution, dichlorofluorescin-diacetate (DCFH-DA), 1 M potassium phosphate buffer solution, horseradish peroxidase (HRP), methanol (HPLC grade), and o-phenylenediamine (≥99.5 %). H2O used for the DCFH, HRP and AA solution was obtained from a Milli-Q high-purity water unit (resistivity ≥18.2MΩcm−1, Merck Millipore, USA). For DTT analysis, 9,10-phenanthrenequinone (PQN) (≥99 %), 5,5’-dithiobis(2-nitrobenzoic acid) (DTNB) (99 %), DL-dithiothreitol (DTT) (≥98 %), potassium phosphate dibasic (≥98 %, Krebs buffer), potassium phosphate monobasic (≥98 %, Krebs buffer) and methanol (≥99.9 %) were all obtained from Fisher Chemical. Nitrogen (oxygen free) was obtained from BOC (Cambridge, UK).
10
Acetylcholinesterase Activity Assay
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Acetylcholinesterase (AChE) from Electrophorus electricus (electrical eels), type VI-S, 200–1000 unit/mg; substrate acetylthiocholine iodide (ATCI); sodium phosphate monobasic; and sodium phosphate dibasic were purchased from Sigma-Aldrich (St. Louis, MO, USA). The coloring agent 5,5-dithio-bis-[2-nitrobenzoic acid] (DTNB) and gallic acid were obtained from Acros (Geel, Belgium). Galantamine hydrobromide was obtained from Calbiochem (San Diego, CA, USA). Indomethacin, 2,2-diphenyl-1-picrylhydrazyl (DPPH), quercetin, zinc powder, and phosphate-buffered saline were also obtained from Sigma-Aldrich (St Louis, MO, USA). Wagner’s, Mayer’s, and Dragendorff’s reagents were obtained from R&M Chemicals (Essex, UK). Folin–Ciocalteu’s reagent and sodium hydroxide were obtained from R&M Chemicals (Essex, UK). Millon’s reagent for the detection of protein and sodium nitrite were obtained from Bendosen Laboratory Chemicals (Bendosen, Norway). Benedict’s solution for reducing sugar was obtained from PC laboratory reagents. Aluminum chloride was obtained from Quality reagent company (Auckland, New Zealand). Ferric chloride and sodium carbonate were obtained from Merck (Darmstadt, Germany). All solvents used were of analytical grade.
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