Mouse monoclonal anti his

Manufactured by Merck Group

Sourced in Germany

Mouse monoclonal anti-His is a laboratory reagent used to detect and purify proteins that have been tagged with a histidine (His) sequence. It is a mouse-derived antibody that specifically binds to the His tag, which is commonly used in recombinant protein expression systems.

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Lab products found in correlation

Hrp conjugated goat anti rabbit igg, by Cell Signaling Technology (1 mentions) Alexa fluor 488 conjugate, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal anti gapdh, by Cell Signaling Technology (1 mentions) Mouse monoclonal anti myc, by Cell Signaling Technology (1 mentions) Mouse monoclonal anti ha, by Cell Signaling Technology (1 mentions) Horseradish peroxidase hrp conjugated goat anti mouse igg, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 594 conjugate, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal anti flag, by Merck Group (1 mentions) Polyvinylidene fluoride (pvdf), by GE Healthcare (1 mentions) Rabbit anti sirt1 h300, by Santa Cruz Biotechnology (1 mentions) Criterion blotter, by Bio-Rad (1 mentions) Goat anti mouse igg hrp, by Santa Cruz Biotechnology (1 mentions) Pvdf membrane, by GE Healthcare (1 mentions) Anti flag m2, by Merck Group (1 mentions)

Close spelling variants of this product

Mouse anti-His monoclonal antibody HRP)-conjugated mouse anti-His monoclonal antibody Mouse anti-His Monoclonal mouse

4 protocols using mouse monoclonal anti his

Comprehensive Immunoblotting and Immunofluorescence Protocols

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The primary antibodies used were: mouse monoclonal anti-Rab2 (Abcam), rabbit polyclonal anti-Sar1 (Abcam), mouse monoclonal anti-GAPDH (Cell Signaling Technology), mouse monoclonal anti-His(Sigma), mouse monoclonal anti-Flag (Sigma), mouse monoclonal anti-Myc (Cell Signaling Technology), mouse monoclonal anti-HA (Cell Signaling Technology), mouse monoclonal anti-GroEL(Santa Cruz) and mouse monoclonal anti-Omp1 (Santa Cruz). The secondary antibodies used for Western blotting were: Horse Radish Peroxidase (HRP)-conjugated Goat Anti-Mouse IgG (Santa Cruz) and HRP-conjugated Goat Anti-Rabbit IgG (Cell Signaling) antibodies. Normal rabbit IgG was purchased from Santa Cruz. The secondary antibodies used for immunofluorescence were: goat anti-rabbit IgG (H + L) secondary antibody, Alexa Fluor^® 488 conjugate (Invitrogen), and donkey anti-rabbit IgG secondary antibody and Alexa Fluor^® 594 conjugate (Invitrogen).

Liu X., Zhou M., Yang Y., Wu J, & Peng Q. (2018). Overexpression of Cu-Zn SOD in Brucella abortus suppresses bacterial intracellular replication via down-regulation of Sar1 activity. Oncotarget, 9(11), 9596-9607.

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SDS-PAGE and Western Blotting of Truncated Proteins

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Truncated proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Briefly, each purified protein was separated by SDS-PAGE under reducing or non-reducing conditions, and then stained with Coomassie blue or transferred to polyvinylidene fluoride membrane (PVDF) (GE Healthcare, Little Chalfont, Bukinghamshire, UK). For immuno-detection, the membrane was saturated with 5% skimmed milk for 2h at room temperature (RT) and incubated with mouse monoclonal anti-His (Sigma-Aldrich, Steinheim, Germany) in 1:12500 dilution for 1h at RT. After washing, the membrane was incubated with horse radish peroxidase (HRP)-conjugated goat anti-mouse IgG in 1:12500 dilution for 1h at RT and proteins were detected with SuperSignal^™ West Pico Chemiluminescent Substrate (Pierce, Thermo Fisher Scientific, Life Technology, Carlsbad, CA, USA).

Zhang X., Bilic I., Marek A., Glösmann M, & Hess M. (2016). C-Terminal Amino Acids 471-507 of Avian Hepatitis E Virus Capsid Protein Are Crucial for Binding to Avian and Human Cells. PLoS ONE, 11(4), e0153723.

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Immunoblot Analysis of NQO1 and SIRT1

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Cell extracts and immunoblot analysis were carried out as previously described (Tsvetkov et al., 2011 (link)). The antibodies used were: goat anti NQO1 C19 and R20 (Santa Cruz), rabbit anti SIRT1 H300 (Santa Cruz) and mouse monoclonal anti His (Sigma).

Tsvetkov P., Adler J., Strobelt R., Adamovich Y., Asher G., Reuven N, & Shaul Y. (2021). NQO1 Binds and Supports SIRT1 Function. Frontiers in Pharmacology, 12, 671929.

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Western Blot of Tagged Proteins

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Tagged proteins (His₆ or FLAG epitope), separated by SDS-PAGE, were transferred to a PVDF membrane (GE Healthcare) using a Criterion blotter (Bio-Rad). The membrane was washed with Western wash buffer (phosphate buffered saline; 0.1% Tween 20) and blocked overnight (with 5% skim milk powder) at 4°C. The membrane was washed 5 × and incubated with the primary antibody for 1 h, washed again (5 ×), incubated with the secondary antibody for 30 min at RT and finally washed again (5 ×). Primary antibodies were mouse monoclonal anti-His (Sigma) or anti-FLAG M2 (Sigma) and the secondary antibody was goat anti-mouse IgG-HRP (Santa Cruz). Proteins were visualized by X-ray film (Kodak) using SignalWest Pico Chemiluminescent substrate kit (Pierce).

Dy R.L., Przybilski R., Semeijn K., Salmond G.P, & Fineran P.C. (2014). A widespread bacteriophage abortive infection system functions through a Type IV toxin–antitoxin mechanism. Nucleic Acids Research, 42(7), 4590-4605.

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