2 n morpholino ethanesulfonic acid mes ph 5.0

The starting materials included polyethylene glycol (M_w: 35 kDa), 2-hydroxy-1-[4-(2-hydroxyethoxy) phenyl]-2-methyl-1-propanone (Irgacure D-2959), lactide, triethylamine, acryloyl chloride, Tin(II) 2-ethylhexanoate [Sn(Oct)₂], dichloromethane (anhydrous), toluene (anhydrous), potassium carbonate (K₂CO₃), calcium chloride (CaCl₂) and magnesium sulfate (MgSO₄) purchased from Sigma-Aldrich (MO, USA), ethyl ether (anhydrous) purchased from Thermo Fisher Scientific (MA, USA), micro-filtered and lyophilized sodium alginate (MVG GRGDSP-coupled; high G ratio; high M_w) purchased from NovaMatrix (Dupont, DE, USA), high purity chitosan (99% degree of deacetylation; M_w: 100 kDa), as primers for tissues, purchased from Sigma-Aldrich (MO, USA), and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and sulfated N-hydroxysuccinimide (Sulfo-NHS), as coupling reagents for tissue and device primers, purchased from Thermo Fisher Scientific (MA, USA). The solvents included ultrapure distilled water, phosphate-buffered saline (PBS; pH 7.4), and 2-(N-morpholino)ethanesulfonic acid (MES; pH 5.0) purchased from Thermo Fisher Scientific (MA, USA). All chemicals were used as received. Tissues and organs for bench studies were purchased from Animal Biotech Industries (ABI; PA, USA) and were cleaned with soap and water before adhesion measurements.