N hydroxysulfosuccinimide sodium salt nhs

All chemicals, including 4-amino-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO, CAS# 14691-88-4), were purchased from Millipore Sigma (St. Louis, MO, USA) and used as received unless otherwise mentioned. The perylene (PY) dye was prepared as previously described [37 (link)]. Dulbecco’s phosphate-buffered saline (DPBS), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1M), Dulbecco’s Modified Eagle Medium (DMEM) containing 25 mM HEPES (DMEM-HEPES), live cell imaging solution (LCIS), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC·HCl, CAS# 25952-53-8), N-hydroxysulfosuccinimide sodium salt (NHS, CAS# 106627-54-7), and the plasma membrane probe wheat germ agglutinin-Alexa Fluor™ 488 conjugate (WGA-AF488) were purchased from Thermofisher Scientific (Waltham, MA, USA). Poly (ethylene glycol) amine hydrochloride (PEG-NH₂.HCl, MW 2000) was purchased from Nanocs Inc. (New York, NY, USA). Human 4-HNE competitive ELISA 96-well assay kit was purchased from LifeSpan Biosciences, Inc. (Seattle, WA, USA). UV-Vis absorbance and fluorescence spectra of the LCNPs were measured on a UV-1900i Spectrophotometer (Shimadzu Scientific Instruments, Columbia, MD, USA) and an RF-6000 Spectrofluorophotometer (Shimadzu Scientific Instruments, Columbia, MD, USA), respectively.

Europium chelate fluorescent microspheres (particle size, 312 nm; excitation wavelength, 365 nm; emission wavelength, 610 nm), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC),s and N-hydroxysulfosuccinimide sodium salt (NHS) were purchased from Thermo Fisher Scientific, USA. Nitrocellulose (NC) membranes (CN140), conjugation pads, sample pads, soleplates, and absorbent paper were purchased from Millipore (Shanghai, China). Goat anti-chicken IgY and chicken IgY antibodies were obtained from Hangzhou Kitgen Biotechnology Co., Ltd., China. The RBD-Ag3, anti-CoV-19 IgG, and ACE2-RP2 proteins were obtained from Fapon Biological Co., Ltd. Carboxyl-functionalized europium chelate nanoparticles (EuNPs) (Catalogue Number 93470720011150) were obtained from Guangzhou Ewell Biotechnology Co. Ltd. All ultrapure water used in this study was obtained from the Milli-Q ultrapure system (Millipore, MA, USA). All chemicals used were pure or higher for analysis.