Huh7 cells were transfected with miR-199a-3p mimic, small interfering RNA for YAP1 (si-YAP1), pcDNA3.1 vectors containing the cDNA of YAP1 (pcDNA-YAP1), pcDNA-Jagged1, si-Jagged1 and their respective controls (Ribobio, Guangzhou, China) by using Lipofactamine 2000 (Invitrogen) according to the manufacturer’s instructions. To investigate whether introduction of miR-199a-3p could increase HCC cell sensitivity to the drug γ-secretase inhibitor n-[n-(3,5-difluorophenacetyl-l-alanyl)]-s-phenylglycinet-butyl ester (DAPT; Calbiochem, San Diego, CA, USA), Huh7 cells transfected with miR-control or miR-199a-3p mimic were treated with DAPT at a final concentration of 10 μM for 48 h.
Pcdna yap1
Manufactured by RiboBio
Sourced in China
PcDNA-YAP1 is a plasmid vector that encodes the human Yes-associated protein 1 (YAP1) gene. YAP1 is a transcriptional regulator involved in the Hippo signaling pathway, which plays a role in cell proliferation and organ size control.
Automatically generated - may contain errors
2 protocols using pcdna yap1
1
Modulating Huh7 Cell Sensitivity to DAPT
2
Regulation of OV90 and SKOV-3 Cells by miR-199a-3p
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OV90 and SKOV-3 cells were grown in 6-well plates to ~80% confluence. Subsequently, 20 nM miR-199a-3p mimics (5′-CCCAGUGUUCAGACUACCUGUUC-3′), mimics negative control (NC) (5′-GUUCCCCAACCUGUGUUCAGACU-3′), miR-199a-3p inhibitor (5′-AACAGGTAGTCTGAACACT-3′) or inhibitor NC (5′-TAACTGACAGGGACACTTA-3′), or 2 µg pcDNA-vector or pcDNA-YAP1 were transfected into cells at 37°C for 24 h using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.). miR-199a-3p mimics, mimics NC, miR-199a-3p inhibitor, inhibitor NC, pcDNA-YAP1 and pcDNA-vector were purchased from Guangzhou RiboBio Co., Ltd. At 48 h post-transfection, the cells were harvested for subsequent experimentation.
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