hMSCs were exposed to adipogenic media (Lonza, Walkersville, MD, USA) in the presence of sucralose (0 mM, 0.2 mM, and 1 mM) for 3 cycles of induction and 3 cycles of maintenance (18 days) followed by gene expression analysis and Oil Red O staining.
Adipogenic media
Manufactured by Lonza
Sourced in United States, Macao
Adipogenic media is a cell culture medium designed to support the differentiation and proliferation of adipocytes, which are the primary cell type found in adipose tissue. The core function of this media is to provide the necessary nutrients, growth factors, and hormones to facilitate the conversion of precursor cells into mature adipocytes.
Automatically generated - may contain errors
Lab products found in correlation
Isobutylmethylxanthine, by Merck Group (2 mentions)
Dexamethasone (dex), by Merck Group (2 mentions)
Indomethacin, by Merck Group (2 mentions)
Osteogenic media, by Lonza (1 mentions)
Pen strep, by Thermo Fisher Scientific (1 mentions)
Bcip nbt, by Merck Group (1 mentions)
Oil red o, by Merck Group (1 mentions)
Trypsin, by Merck Group (1 mentions)
Antibiotic antimycotic solution, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
4 protocols using adipogenic media
1
Adipogenic Differentiation of hMSCs
2
Multimodal Imaging of Stem Cell Differentiation
3
Adipogenic Differentiation of HS-5 Cells
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HS-5, human bone marrow stromal cells, were purchased from American Type Culture Collection (Rockville, MD, USA). After thawing, HS-5 cells were resuspended in DMEM, supplemented with 10% heat inactivated fetal bovine serum (FBS, Invitrogen, Carlsbad, CA, USA) and 1% antibiotic/antimycotic solution (Invitrogen), and plated in a 75 cm2 flask at a density of 1 to 2 × 104 cells. The medium was replaced with adipogenic medium, and the cells were cultured for additional 19 days. The adipogenic media (Lonza, Basel, SW) consisted of complete culture medium supplemented with DMEM-high glucose (4.5 g/L), 10% (v/v) FBS, 10 μg/ml insulin, 0.5 mM dexamethasone (Sigma-Aldrich, St. Louis, MO), 0.5 mM isobutylmethylxanthine (Sigma-Aldrich, St. Louis, MO), and 0.1 mM indomethacin (Sigma– Aldrich, St. Louis, MO). Medium was changed every 3 days. In our experiments human BMSCs were cultured in the presence of NAC (10 mM) which was added once for the last 3 days of differentiation.
4
Adipogenic Differentiation of ASCs
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ASCs (passages 4 to 5) were plated in a 75 cm2 flask at a density of 1 to 2 × 104 cells and cultured in DMEM with 10% FBS for 7 days. The medium was replaced with adipogenic medium, and the cells were cultured for additional 14 or 21 days.
The adipogenic media (Lonza, Basel, SW) consisted of complete culture medium supplemented with DMEM-high glucose, 10% (v/v) FBS, 10 μg/mL insulin, 0.5 mM dexamethasone (Sigma-Aldrich, St. Louis, MO), 0.5 mM isobutylmethylxanthine (Sigma-Aldrich, St. Louis, MO), and 0.1 mM indomethacin (Sigma-Aldrich, St. Louis, MO). Media were changed every 3 days. Our preliminary results on ASCs demonstrate no significant differences in cell viability between 5, 10, and 25 μM of Cape. In our experiments human ASCs were cultured in the presence of Cape (10 μM) which was administered every 3 days in the first set of experiments while for the second set of experiments Cape was added once for the last 3 days of differentiation at the same concentration. Additionally, ASC-derived adipocytes were cultured in adipogenic differentiation media and LPS was added 6 hrs before collecting cells at a dose of 1 ng/mL.
The adipogenic media (Lonza, Basel, SW) consisted of complete culture medium supplemented with DMEM-high glucose, 10% (v/v) FBS, 10 μg/mL insulin, 0.5 mM dexamethasone (Sigma-Aldrich, St. Louis, MO), 0.5 mM isobutylmethylxanthine (Sigma-Aldrich, St. Louis, MO), and 0.1 mM indomethacin (Sigma-Aldrich, St. Louis, MO). Media were changed every 3 days. Our preliminary results on ASCs demonstrate no significant differences in cell viability between 5, 10, and 25 μM of Cape. In our experiments human ASCs were cultured in the presence of Cape (10 μM) which was administered every 3 days in the first set of experiments while for the second set of experiments Cape was added once for the last 3 days of differentiation at the same concentration. Additionally, ASC-derived adipocytes were cultured in adipogenic differentiation media and LPS was added 6 hrs before collecting cells at a dose of 1 ng/mL.
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