Highly purified lipids such as 1,2-dipalmitoyl-Sn-glycero-3-phosphocholine (DPPC), cholesterol were bought from the Avanti Polar Lipids (Alabaster, AL, USA). Cytokines and antioxidants estimation kits were obtained from the Abcam (Cambridge, UK). Whereas Ellagic acid, Cyclophosphamide and RPMI were from MedChem Express (Monmouth Junction, NJ, USA). Nutrient Agar and Tryptic soya broth (TSB) were procured from the HiMedia Company (Mumbai, India).
Cyclophosphamide
Manufactured by MedChemExpress
Sourced in United States
Cyclophosphamide is a chemical compound used as a laboratory reagent. It is a white, crystalline solid that is soluble in water and organic solvents. Cyclophosphamide is commonly used in research applications as a chemical agent.
Automatically generated - may contain errors
Lab products found in correlation
Ellagic acid, by MedChemExpress (1 mentions)
Nutrient agar, by HiMedia (1 mentions)
Tryptic soya broth, by HiMedia (1 mentions)
1 2 dipalmitoyl sn glycero 3 phosphocholine dppc cholesterol, by Avanti Polar Lipids (1 mentions)
Fm4 64fx, by Thermo Fisher Scientific (1 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride edc hcl, by GL Biochem (1 mentions)
1 hydroxybenzotriazole hobt, by GL Biochem (1 mentions)
Fluorescein isothiocyanate (fitc), by Biotium (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Thermo Fisher Scientific (1 mentions)
Mcl 1 sc 12756, by Santa Cruz Biotechnology (1 mentions)
Alexa fluor 488 donkey anti mouse a32766, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Carboplatin, by MedChemExpress (1 mentions)
Vincristine, by MedChemExpress (1 mentions)
Capecitabine, by MedChemExpress (1 mentions)
Gemcitabine, by MedChemExpress (1 mentions)
Pomalidomide, by MedChemExpress (1 mentions)
Lenalidomide, by MedChemExpress (1 mentions)
Ab2907, by Abcam (1 mentions)
Bortezomib, by MedChemExpress (1 mentions)
5 protocols using cyclophosphamide
1
Lipid-based Antioxidant and Cytokine Assay Protocol
2
Synthesis and Characterization of Novel Biomaterials
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All chemicals used in the syntheses unless otherwise specified were purchased from Sigma-Aldrich Co. LLC. l -lysine and DiS-C3-(5) were purchased from Combi-Blocks, Inc. N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC.HCl) and 1-hydroxybenzotriazole (HOBt) were purchased from GL Biochem Ltd. Cyclophosphamide was purchased from MedChemExpress LLC. PI and FM 4–64FX were purchased from Thermo Fisher Scientific. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Alfa Aesar. FITC was purchased from Biotium, Inc. Technical grade solvents were purchased from SG Labware Pte Ltd. (Singapore) and directly used for column purification without any distillation. Pullulan standards were purchased from Polymer Standards Service.
3
Chemotherapeutic Drugs Evaluation Protocol
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Chemotherapeutic drugs, VP-16, DXR, PTX, CDDP, carboplatin, gemcitabine, 5-FU, cyclophosphamide, capecitabine, and vincristine were purchased from MedChemExpress. Topo IIα (catalog 12286), Smurf2 (catalog 12024), cPARP (catalog 5625), and Bim (catalog 2933) antibodies were purchased from Cell Signaling Technology Inc. Mcl-1 (sc-12756) and Topo IIβ (sc-55330) antibodies were purchased from Santa Cruz Biotechnology. Anti–phospho-histone H2AX (Ser139; γ-H2AX) antibody was purchased from MilliporeSigma (catalog 05–636). DAPI (catalog 62248) and secondary antibody Alexa Fluor 488 donkey anti-mouse (A32766) were purchased from Thermo Fisher Scientific. Other reagents and antibodies were the same as described previously (10 (link), 16 (link)).
4
Immune-Deficient Xenograft Tumor Model
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Mice were operated and housed according to the protocols approved by the Ethics Committee of Guilin Medical University. Briefly, 200 μl cyclophosphamide (Cat # HY-17420, MedChemExpress, Monmouth Junction, NJ, USA) was injected for three consecutive days to further destroy the immune system of the nude mice. Then mice were injected at a density of 1×107, 1×106, and 1×105 cells/tumor, respectively. Nude mice were euthanized with isoflurane after 10 days and tumors were separated. The ratio of stem cells was calculated using an ELDA: Extreme Limiting Dilution Analysis (http://bioinf.wehi.edu.au/software/elda/ ).
5
Evaluating C1q Receptor Expression
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WB was performed as previously described (17 (link)). Each experiment was repeated at least three times, with a representative experiment shown. The antibodies were as follows: anti-gC1qR antibody (Abcam, ab24733), anti-cC1qR antibody (Abcam, ab2907), anti-IGF2BP3 antibody (Abcam, ab177477), anti-β-actin antibody (Cell Signaling Technology, 4967S), and secondary horseradish-peroxidase-conjugated antibodies (Abcam, ab205719, ab205718). Five clinical drugs (bortezomib, ixazomib, lenalidomide, pomalidomide, and cyclophosphamide) were purchased from Med Chem Express, USA.
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