Anti nur77 m 210

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-Nur77 (M-210) is a monoclonal antibody that recognizes the Nur77 protein. Nur77 is a transcription factor that plays a role in various cellular processes. This antibody can be used for the detection and study of Nur77 expression in various experimental systems.

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Lab products found in correlation

Anti hsp60, by Abcam (2 mentions) Anti ubiquitin, by Abcam (1 mentions) D galn, by Merck Group (1 mentions) Anti lc3, by Cell Signaling Technology (1 mentions) Anti lc3b, by Santa Cruz Biotechnology (1 mentions) Elisa kits for il 1β and il 6, by Elabscience (1 mentions) Anti myc 9e10, by Abcam (1 mentions) Anti p65, by Santa Cruz Biotechnology (1 mentions) Anti gst, by Santa Cruz Biotechnology (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Anti iκbα, by Cell Signaling Technology (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Jc 1 probe, by Thermo Fisher Scientific (1 mentions) Mitosox red mitochondrial superoxide indicator, by Thermo Fisher Scientific (1 mentions) Anti pcna, by Santa Cruz Biotechnology (1 mentions) Anti parp, by Santa Cruz Biotechnology (1 mentions) Anti bcl 2, by Santa Cruz Biotechnology (1 mentions) Anti nur77, by Cell Signaling Technology (1 mentions) Mitotracker deep red, by Merck Group (1 mentions) Sybr green master mixture, by Roche (1 mentions)

3 protocols using anti nur77 m 210

Investigating Nur77-mediated Inflammation

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Anti-Myc (9E10) (Cat. Sc-40), and anti-Hsp60 (Cat. ab46798) antibodies from Abcam (UK); anti-Ubiquitin (Cat. 3933S), anti-β-actin (Cat. 4970S), anti-IκBα (Cat.4814S), and anti-LC3 (Cat. 4108S) antibodies from Cell Signal Technology (Beverly, MA, USA); anti-Nur77 (M-210) (Cat. sc-5569), anti-LC3B (Santa Cruz sc-28266), anti-p65 (Santa Cruz sc-109) and anti-GST (Cat.sc-138) antibodies from Santa Cruz Biotechnology (Santa Cruz, CA, USA); mouse IL-1β (interleukin 1β, Cat. E-EL-M0037c) and IL-6 (interleukin 6, Cat. E-EL-M0044c) ELISA kits for IL-1β and IL6 from Elabscience Biotechnology (Wuhan, Hubei, China); LPS (Lipopolysaccharide, Cat. L2630) and D-Gal N (D-Galactosamine, Cat. G0500) from Sigma (St. Louis, MO, USA); TianScript RT kit (Cat. KR104-02) from TianGen Biotechnology (Beijing, China); and Nur77, RXRα, and control siRNAs from Sigma were used.

Hu M., Luo Q., Alitongbieke G., Chong S., Xu C., Xie L., Chen X., Zhang D., Zhou Y., Wang Z., Ye X., Cai L., Zhang F., Chen H., Jiang F., Fang H., Yang S., Liu J., Diaz-Meco M.T., Su Y., Zhou H., Moscat J., Lin X, & Zhang X.K. (2017). Celastrol-Induced Nur77 Interaction with TRAF2 Alleviates Inflammation by Promoting Mitochondrial Ubiquitination and Autophagy. Molecular cell, 66(1), 141-153.e6.

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Multiparametric Evaluation of Mitochondrial Function

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Anti-Myc (9E10) (Cat. Sc-40), anti-Ki67 (Cat. ab15580) and anti-Hsp60 (Cat. ab46798) antibodies were purchased from Abcam (UK); anti-β-actin (Cat. 4970S), anti-Cleaved caspase-3 (Cat. 9661S), and anti-Nur77 (Cat. 3960S) antibodies were purchased from Cell Signal Technology (Beverly, MA, USA); anti-Nur77 (M-210) (Cat. sc-5569), anti-PCNA (Santa Cruz sc-7907), anti-a-tublin (Santa Cruz sc-8035), anti-Bcl-2 (Santa Cruz sc-783), anti-PARP (Santa Cruz sc-7150), and anti-GST (sc-138) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA); anti-Flag (Cat.F1804) antibody was purchased from Sigma (St. Louis, MO, USA); Mito-tracker deep red (Cat. M22426), JC-1 Probe (Cat. T3168) and mitoSOX Red Mitochondrial Superoxide Indicator (Cat. M36008) were purchased from Thermo Fisher.

Chen X., Cao X., Tu X., Alitongbieke G., Xia Z., Li X., Chen Z., Yin M., Xu D., Guo S., Li Z., Chen L., Zhang X., Xu D., Gao M., Liu J., Zeng Z., Zhou H., Su Y, & Zhang X.K. (2019). BI1071, a novel Nur77 modulator, induces apoptosis of cancer cells by activating the Nur77-Bcl-2 apoptotic pathway. Molecular cancer therapeutics, 18(5), 886-899.

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Nur77 Binding Site Identification

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(2 × 10⁷) CD8⁺ T-cells isolated from peripheral lymph nodes using a CD8⁺selection kit (EasySep) were activated with plate bound anti-CD3, CD28 overnight. CD8⁺cells were then transfected with Flag-tagged Nr4a1 and cross-linked with 2 mM DSG (Sigma) and 1% formaldehyde methanol-free (Thermo Scientific Pierce). Cells were then lysed in RIPA buffer with protease inhibitors (Sigma), and chromatin was sheared by sonication in a Bioruptor sonicator (Diagenode). Immunoprecipitation was carried out with Dynabeads Protein A (Life Technologies) and Anti-Flag antibody (Origene). DNA was isolated using a ChIP DNA Clean and Concentrator column (Zymo). Target genes were amplified from the isolated DNA using SYBR® green master mixture (Roche). anti-Nur77 (M-210) and Anti-CoREST (Santa Cruz) was used for IP.

Nowyhed H.N., Huynh T.R., Blatchley A., Wu R., Thomas G.D, & Hedrick C.C. (2015). The Nuclear Receptor Nr4a1 Controls CD8 T Cell Development Through Transcriptional Suppression of Runx3. Scientific Reports, 5, 9059.

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