For checking the c-fos expression in dark, mice were taken from the animal facility before the light on and perfused under the red light with PBS and 4% PFA. For checking the c-fos expression in laboratory ambient light, mice were taken from the animal facility 4 hours after the light on and perfused with PBS and 4% PFA. Brains were dissected out and sliced in 50pm-thickness slices with vibratome (Leica VT1000S). The light intensity was measured using light meter (Fisher Scientific). We used c-fos antibody (Cell Signaling Technology, #2250 1:500) to perform the immunostaining and images were taken using confocal microscope (FV1000, Olympus).
Light meter
Manufactured by Thermo Fisher Scientific
Sourced in United States
A light meter is a device used to measure the intensity or illuminance of light. It provides a quantitative measurement of the amount of light present in a specific environment or on a surface. The core function of a light meter is to accurately assess the level of ambient light, which is essential for various applications such as photography, lighting design, and energy efficiency assessments.
Automatically generated - may contain errors
2 protocols using light meter
1
Quantifying c-fos Expression in Mouse Brain
2
Fundus Fluorescein Angiography in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were dark adapted overnight in a darkroom equipped with a red light source during the experiment. Before applying light to each eye, the light intensity from the Micron IV mouse fundus camera was measured using a light meter (Fisher Scientific, Hampton, NH, USA; Cat # S90199) to ensure that equal illumination was provided to all eyes. Each mouse was anesthetized, one at a time, and the pupils dilated as stated above. We applied GenTeal gel to eyes to prevent corneal dryness during the length of the experiment. A single intraperitoneal injection of fluorescein (100 µL of a 1:5 diluted 10% fluorescein solution) was administered just before the camera was centered on the optic disc head and focused on the RPE. The desired light intensity was then applied to the retina as described previously.34 (link) In brief, after a 4-minute delay after the fluorescein injection the light intensity was increased to 45K lux and continued for 3 minutes (a protocol we called FA 3@4) while monitoring that no changes happen in the fundus orientation during this time. Mice were kept under normal lighting conditions after the light procedure.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!