Calibrate beads

Manufactured by BD

Sourced in Germany

Calibrate beads are spherical particles used to calibrate and verify the performance of flow cytometers. They are designed to provide accurate size and fluorescence intensity references for instrument setup and quality control.

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Lab products found in correlation

Lsrfortessa 1, by BD (1 mentions) Streptavidin pe texas red, by Thermo Fisher Scientific (1 mentions) Fluoro gold, by Merck Group (1 mentions) Anti hla dr, by BD (1 mentions) Anti cd163, by R&D Systems (1 mentions)

Close spelling variants of this product

BD Calibrate 3 Beads (2 citations)

2 protocols using calibrate beads

Multiparametric Flow Cytometry Analysis

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Organ cellularity was determined by counting of cell suspensions from bone marrow, spleen, and thymus with calibrate beads (BD Biosciences). Cells were stained on ice for 30 minutes, washed once, and then incubated with streptavidin–PE–Texas red (Life Technologies). After a washing step, acquisition was performed on an LSR Fortessa 1 (BD Biosciences) and analyzed with FlowJo (Tree Star). Antibodies for flow cytometry either were labeled in house with FITC, PE, PECy7, APC, or Alexa Fluor 700 or were biotinylated. CD4 (GK1.5), CD8 (53.6.7), CD16/32 (24G2), CD19 (1D3), CD41 (MWReg30), Ter119 (Ly76), Gr1 (RB6-8C5), CD11b (M1/70), Sca1 (D7), Kit (ACK4), B220 (RA3-6B2), and CD34-FITC (RAM34) were purchased from BD Pharmingen, and CD150-PE (TC15-12F12.2) was purchased from BioLegend. Fluorogold (Sigma-Aldrich) was used to exclude dead cells.

Pleines I., Woods J., Chappaz S., Kew V., Foad N., Ballester-Beltrán J., Aurbach K., Lincetto C., Lane R.M., Schevzov G., Alexander W.S., Hilton D.J., Astle W.J., Downes K., Nurden P., Westbury S.K., Mumford A.D., Obaji S.G., Collins P.W., Delerue F., Ittner L.M., Bryce N.S., Holliday M., Lucas C.A., Hardeman E.C., Ouwehand W.H., Gunning P.W., Turro E., Tijssen M.R, & Kile B.T. (2017). Mutations in tropomyosin 4 underlie a rare form of human macrothrombocytopenia. The Journal of Clinical Investigation, 127(3), 814-829.

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Hepatic Leukocyte Isolation and Analysis

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For human macrophages, cell surface markers were stained with directly conjugated mouse anti-human antibodies MRP8/14 (BMA biomedicals, Switzerland), anti-CD163 (R&D systems, USA) and anti-HLA-DR (BD, Germany). For mouse experiments, hepatic leukocytes were isolated and stained for flow cytometry as described earlier.[27 (link)] Cell suspensions were filtered using a 100 μm mesh. To count cells, 2x10⁴ counting beads (calibrate beads, BD, Germany) were added to all samples. Flow cytometric data are given as MFI. Human blood cell populations are represented in absolute numbers or as percentages of leukocytes. Murine cell populations are shown in absolute numbers calculated from organ weight or as percentages of leukocytes. Using this methodology for calculation, a healthy mouse liver (~2 g) contains approximately 1.25 million Kupffer cells.

Warzecha K.T., Bartneck M., Möckel D., Appold L., Ergen C., Al Rawashdeh W., Gremse F., Niemietz P.M., Jahnen-Dechent W., Trautwein C., Kiessling F., Lammers T, & Tacke F. (2018). Targeting and Modulation of Liver Myeloid Immune Cells by Hard-Shell Microbubbles. Advanced biosystems, 2(5), 1800002.

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