Anti mouse and anti rabbit hrp conjugated secondary antibodies

Manufactured by Merck Group

Anti-mouse and anti-rabbit HRP-conjugated secondary antibodies are laboratory reagents used to detect and visualize target proteins in various immunoassays, such as Western blotting, ELISA, and immunohistochemistry. These antibodies are conjugated with horseradish peroxidase (HRP), an enzyme that catalyzes a colorimetric or chemiluminescent reaction, allowing for the detection and quantification of the target proteins.

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Lab products found in correlation

Immobilon p pvdf membrane, by Merck Group (2 mentions) Luminata forte western hrp substrate, by Merck Group (2 mentions) Mouse anti gapdh, by Santa Cruz Biotechnology (2 mentions)

Close spelling variants of this product

HRP-conjugated goat anti-rabbit and anti-mouse secondary antibodies HRP-conjugated anti-mouse and anti-rabbit antibodies Anti-rabbit and anti-mouse secondary antibodies Anti-mouse and anti-rabbit HRP antibodies Anti-rabbit HRP antibodies Anti-mouse secondary antibodies HRP-conjugated antibodies Mouse secondary antibodies Anti-rabbit antibodies Anti-mouse antibodies

2 protocols using anti mouse and anti rabbit hrp conjugated secondary antibodies

Western Blot Analysis of HBV Core Protein

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Example 8

Cells were lysed by addition of RIPA buffer [50 mM Tris (pH 7.5), 150 mM NaCl, 1 mM EGTA, 1 mM EDTA, 0.1% SDS, 1% Triton X-100] and incubated on ice for 30 min with occasional vortexing. The lysate was centrifuged at 14,000×g for 15 min, the supernatant was collected, and protein was quantitated by Bradford assay. Protein (8 μg) in laemmli buffer was separated by SDS-PAGE and transferred to PVDF Immobilon-P membranes (Millipore, Billerica, Mass.). Membranes were probed with rabbit anti-HBV core (Austral Biologicals, San Ramon, Calif.) (1:500 dilution) and mouse anti-GAPDH (Santa Cruz Biotechnology) (1:5,000) antibodies, followed by anti-mouse and anti-rabbit HRP-conjugated secondary antibodies (Sigma-Aldrich). Bound antibodies were visualized by adding Luminata Forte Western HRP substrate (Millipore) to the membrane and imaged with a Fuji camera system.

US10759774B2. Inhibitors of hepatitis B virus targeting capsid assembly (2020-09-01). THE CURATORS OF THE UNIVERSITY OF MISSOURI [US], REGENTS OF THE UNIVERSITY OF MINNESOTA [US]. Inventors: Stefan G. Sarafianos [US], Zhengqiang Wang [US], Andrew D. Huber [US], Jing Tang [US].

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HBV Core Protein Western Blot Protocol

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Cells were lysed by addition of RIPA buffer [50 mM Tris (pH 7.5), 150 mM NaCl, 1 mM EGTA, 1 mM EDTA, 0.1% SDS, 1% Triton X-100] and incubated on ice for 30 min with occasional vortexing. The lysate was centrifuged at 14,000xg for 15 min, the supernatant was collected, and protein was quantitated by Bradford assay. Protein (8 μg) in laemmli buffer was separated by SDS- PAGE and transferred to PVDF Immobilon-P membranes (Millipore). Membranes were probed with rabbit anti-HBV core (Austral Biologicals) (1:500 dilution) and mouse anti-GAPDH (Santa Cruz Biotechnology) (1:5,000) antibodies, followed by anti-mouse and anti-rabbit HRP- conjugated secondary antibodies (Sigma-Aldrich). Bound antibodies were visualized by adding Luminata Forte Western HRP substrate (Millipore) to the membrane and imaging with a Fuji camera system.

Huber A.D., Pineda D.L., Liu D., Boschert K.N., Gres A.T., Wolf J.J., Coonrod E.M., Tang J., Laughlin T.G., Yang Q., Puray-Chavez M.N., Ji J., Singh K., Kirby K.A., Wang Z, & Sarafianos S.G. (2019). Novel Hepatitis B Virus Capsid-Targeting Antiviral that Aggregates Core Particles and Inhibits Nuclear Entry of Viral Cores. ACS infectious diseases, 5(5), 750-758.

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