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Mouse anti hcv ns5a monoclonal antibody

Manufactured by Merck Group
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The Mouse anti-HCV NS5A monoclonal antibody is a laboratory tool used for the detection and analysis of the hepatitis C virus (HCV) non-structural protein 5A (NS5A). This antibody is specific to the NS5A protein and can be used in various immunological techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to help researchers study the HCV virus and its components.

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Lab products found in correlation

Rabbit anti p62 antibody, by Cell Signaling Technology (2 mentions) M per mammalian protein extraction reagent, by Thermo Fisher Scientific (2 mentions) Rabbit anti atg5 antibody, by Cell Signaling Technology (1 mentions) Rabbit anti lc3 antibody, by Merck Group (1 mentions) Er tracker blue, by Thermo Fisher Scientific (1 mentions) Rabbit anti calnexin antibody, by Abcam (1 mentions)

2 protocols using mouse anti hcv ns5a monoclonal antibody

1

Immunoblotting of Autophagy Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
The primary antibodies used in this study included the mouse anti-STX7 antibody (Sigma-Aldrich), rabbit anti-ATG5 antibody (Cell Signaling), rabbit anti-p62 antibody (Cell Signaling), mouse anti-HCV NS5A monoclonal antibody (Millipore), rabbit anti-LC3 antibody (Sigma-Aldrich), and rabbit anti-calnexin antibody (Abcam). Proteins were extracted from cell lysates for western-blot analysis using the M-PER mammalian protein extraction reagent (Thermo Fisher Scientific) following the manufacturer’s protocol. The ER Tracker Blue was purchased from Thermo Fisher Scientific.
Wang L., Kim J.Y., Liu H.M., Lai M.M, & Ou J.H. (2017). HCV-induced autophagosomes are generated via homotypic fusion of phagophores that mediate HCV RNA replication. PLoS Pathogens, 13(9), e1006609.
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2

Immunoblotting of Autophagy and HCV Proteins

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The primary antibodies used in this study included the rabbit anti-Rubicon antibody (Abcam), rabbit anti-UVRAG antibody (Sigma-Aldrich), rabbit anti-p62 antibody (Cell Signaling), mouse anti-HCV NS5A monoclonal antibody (Millipore), rabbit anti-LC3 antibody (MBL), and rabbit anti-core antibody [45 (link)]. Proteins were extracted from cell lysates for Western blotting using the M-PER mammalian protein extraction reagent (Thermo Scientific) following the manufacturer’s protocol.
Wang L., Tian Y, & Ou J.H. (2015). HCV Induces the Expression of Rubicon and UVRAG to Temporally Regulate the Maturation of Autophagosomes and Viral Replication. PLoS Pathogens, 11(3), e1004764.
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