MET was purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China) and dissolved in phosphate-buffered saline (PBS) as a stock solution of 2 M. The NO prodrug JS-K was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) and dissolved in dimethyl sulfoxide (DMSO) as a stock solution of 5 mM. N-acetylcysteine (NAC) and glutathione disulfide (GSSG) were obtained from Beyotime Institute of Biotechnology (Shanghai, China) and dissolved in PBS to concentrations of 100 mM and 5 mM respectively. All stock solutions were stored at -20°C for further use. Antibodies against Bak, Bcl-2-associated X protein, B-cell lymphoma 2, caspase-3, caspase-9, cytochrome c (Cyto-C), Phosphorylated histone H2AX (γH2AX), DNA repair protein Rad51, and Proliferating cell nuclear antigen (PCNA) were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA), and antibody against GAPDH was purchased from Abcam (Cambridge, UK). Horseradish peroxidase-conjugated IgG secondary antibodies were purchased from EarthOx Life Sciences (Millbrae, CA, USA).
Glutathione disulfide gssg
Manufactured by Beyotime
Sourced in China
Glutathione disulfide (GSSG) is a chemical compound that serves as an important oxidized form of the antioxidant glutathione. It is a key component in cellular redox regulation and plays a crucial role in maintaining the balance between oxidation and reduction processes within cells.
Automatically generated - may contain errors
2 protocols using glutathione disulfide gssg
1
Anticancer Agents and Cellular Responses
2
Apoptosis Signaling Pathway Assay
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MET and PTX were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). MET was dissolved in 1X PBS to a concentration of 2 M, and PTX was dissolved in 100% dimethyl sulfoxide (DMSO) to create a 10 mM stock solution; these were stored at −20°C. N-acetylcysteine (NAC) and glutathione disulfide (GSSG) were purchased from Beyotime Institute of Biotechnology. NAC (100 mM) and GSSG (10 mM) in PBS stock solutions were stored at −20°C. Antibodies against poly (ADP-ribose) polymerase (PARP; cat. no. 9542), caspase-3 (cat. no. 9665), caspase-9 (cat. no. 9502), B-cell lymphoma 2 (Bcl-2; cat. no. 2872), Bcl-2-associated X protein (Bax; cat. no. 2772), cytochrome c (Cyto-C; cat. no. 11940) and P53 (cat. no. 9284p) were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). GAPDH (cat. no. ab37168) antibody was purchased from Abcam (Cambridge, UK). Immunoglobulin G-horseradish peroxidase (IgG-HRP; cat. no. 030181) was purchased from EarthOx Life Sciences (Millbrae, CA, USA).
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