Gibco dulbecco s modified eagle medium nutrient mixture f12 dmem f12

Manufactured by Thermo Fisher Scientific

Sourced in United States

Gibco Dulbecco's Modified Eagle Medium: nutrient mixture F12 (DMEM/F12) is a balanced salt solution that provides a basic formulation of amino acids, vitamins, salts, and other components to support the growth and maintenance of various cell types in cell culture applications.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions) Anti vimentin anti cytokeratin 19 anti bodies, by Merck Group (1 mentions) Tetrachloroauric 3 acid trihydrate, by Merck Group (1 mentions) Penicillin g, by Merck Group (1 mentions) Amphotericin b, by Merck Group (1 mentions) Streptomycin, by Merck Group (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Acetic acid, by Merck Group (1 mentions) Horse serum, by Thermo Fisher Scientific (1 mentions) Nk 92 cells, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by R&D Systems (1 mentions) Recombinant il 2, by R&D Systems (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Antibiotic antimycotic solution, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Gibco Dulbecco’s Modified Eagle Medium Gibco Dulbecco’s modified Eagle’s medium Gibco Dulbecco modified Eagle medium DMEM): nutrient mixture F12 medium DMEM/F12 nutrient mixture DMEM-F12 GIBCO Gibco DMEM medium DMEM/F12 medium Dulbecco’s modified F12 nutrient

4 protocols using gibco dulbecco s modified eagle medium nutrient mixture f12 dmem f12

Chitosan-Mediated Gold Nanoparticle Synthesis

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Tetrachloroauric (III) acid trihydrate (HAuCl₄·3H₂O, 99.9%), acetic acid (CH₃COOH, 99.5%), and ascorbic acid (C₆H₈O₆, 99.7%) were purchased from Merck (Darmstadt, Germany). Chitosan (CS, (C₁₂H₂₄N₂O₉)_n, >75% deacetylated, M_w 310 000–375 000 Dalton) was purchased from Sigma-Aldrich (St. Louis, MO, USA), and used without post-modification. Collagenase Type 1A, fetal bovine serum (FBS), l-glutamine, (4-(2-hydroxyethyl) piperazine-1-ethane sulfonic acid) (HEPES. 99.5%), anti-vimentin/anti-cytokeratin 19 anti-bodies, amphotericin B, penicillin G, and streptomycin were obtained from Sigma-Aldrich (St. Louis, Missouri, USA). Gibco Dulbecco's Modified Eagle Medium: nutrient mixture F12 (DMEM/F12) was purchased from Thermo Fisher Scientific (Waltham, MA, USA).
Chemicals are analytical grade and used without further purification, and the deionized water (conductivity below 4.3 μS cm⁻¹) was used in all the aqueous phase experiments.

Nguyen T.A., Kim Do A.N., Hoang Lo T.N., Park I, & Vo K.Q. (2022). Single-step controlled synthesis of flower-like gold nanoparticles stabilized by chitosan for sensitive detection of heparin using a surface-enhanced Raman scattering method. RSC Advances, 12(54), 34831-34842.

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Establishment and Culturing of CCA and NK-92 Cells

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CCA cell lines including KKU055 (JCRB1551), KKU100 (JCRB1568), and KKU213A (JCRB1557) cells were originated from the CCA patients residing in opisthorchiasis endemic areas in the Northeastern Thailand. These cell lines were established by the researchers at the Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, and deposited at the Japanese Collection of Research Bioresources Cell Bank (JCRB Cell Bank), where we obtained for our studies. The CCA cells were cultured in Gibco Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12) (Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C under a humidified 5% CO₂. NK-92 cells obtained from ATCC (ATCC, Manassas, VA, USA) were cultured in Minimum Essential Medium Eagle-α Modification supplemented with 12.5% horse serum (Thermo Fisher Scientific, Waltham, MA, USA), 2 mM L-glutamine, 0.02 mM inositol, 0.1 mM 2-mercaptoethanol, 0.02 mM folic acid, 100–200 U/mL recombinant IL-2 (R & D system, Minneapolis, MN, USA) and 12.5% fetal bovine serum.

Chiawpanit C., Panwong S., Sawasdee N., Yenchitsomanus P.T, & Panya A. (2022). Genistein Sensitizes Human Cholangiocarcinoma Cell Lines to Be Susceptible to Natural Killer Cells. Biology, 11(8), 1098.

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Isolation and Culture of cBM-MSCs

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For cBM-MSCs isolation, the aspirated bone marrow was mixed with 10 mL Hank's Balanced Salt Solution (HBSS) (Thermo Fisher Scientific Corporation, USA) as a washing solution. After centrifugation at 300 rpm for 15 min, the supernatant, solution above the white ring layer lied over red blood cell, was gently discarded. The second wash with 20 mL HBSS was performed at 1,000 rpm for 15 min, and the supernatant was gently discarded. The pellet was gently resuspended with 10 mL Gibco™ Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F-12) (Thermo Fisher Scientific Corporation, USA) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific Corporation, USA), 2 mM L-glutamine, and 1% Antibiotic-Antimycotic solution (Thermo Fisher Scientific Corporation, USA). Cells were seeded in T-75 Corning® treated tissue culture flasks (Corning, USA) containing pre-warmed culture medium and subsequently incubated under 5% CO₂ and 95% air at 37°c condition. The culture medium, 10% FBS-DMEM/F12, was replaced every 48 h. Cells were subcultured once the confluence reached 80% using 0.25% trypsin-ethylenediaminetetraacetic acid (EDTA) (Thermo Fisher Scientific Corporation, USA). Cells in passage 2–5 were used for the experiments.

Nantavisai S., Rodprasert W., Pathanachai K., Wikran P., Kitcharoenthaworn P., Smithiwong S., Archasappawat S, & Sawangmake C. (2019). Simvastatin enhances proliferation and pluripotent gene expression by canine bone marrow-derived mesenchymal stem cells (cBM-MSCs) in vitro. Heliyon, 5(10), e02663.

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Cultivation of Thai CCA Cell Lines

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Human CCA cell lines, KKU-213A, KKU-100 and KKU-055 were a kind gift from the Faculty of Medicine, Siriraj Hospital, Mahidol University, Thailand. The cells were purchased from the Japanese Collection of Research Bioresources Cell Bank (JCRB, National Institute of Biomedical Innovation, Osaka, Japan). KKU-213A, KKU-100 and KKU-055 were originally established from primary tumors of Thai CCA patients in which KKU-213A [26 (link)] was described as poorly differentiated squamous cell carcinoma whereas KKU-100 and KKU-055 was characterized as poorly differentiated adenocarcinoma [52 (link)]. The cells were cultured in Gibco Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F-12) (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific) and incubated 37 °C, 5% CO₂ incubator.

Panwong S., Wathikthinnakon M., Kaewkod T., Sawasdee N., Tragoolpua Y., Yenchitsomanus P.T, & Panya A. (2021). Cordycepin Sensitizes Cholangiocarcinoma Cells to Be Killed by Natural Killer-92 (NK-92) Cells. Molecules, 26(19), 5973.

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