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Gradient water purification system

Manufactured by Merck Group
Sourced in Germany

The Gradient water purification system is a laboratory equipment designed to produce high-quality water for various scientific and research applications. Its core function is to remove impurities and contaminants from water through a multi-step filtration process, providing a consistent and reliable supply of purified water.

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3 protocols using gradient water purification system

1

Fabrication of PEG-Functionalized Gold Substrates

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Poly(ethylene glycol) (PEG) thiol (MW 550 Da) was purchased from Creative PEGWorks. Tris (2-carboxyethyl) phosphine hydrochloride (TCEP), 2-(N-morpholino)ethanesulfonic acid (MES) hydrate, hydrogen peroxide solution, ethyl alcohol (200 proof), mucin from porcine stomach and 11-mercaptoundecanoic acid (MUA) were purchased from Sigma-Aldrich. EDC (1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride) and NHS (N-hydroxysuccinimide) were purchased from Thermo Fisher Scientific. Silicon wafers, 76.2 mm diam., were purchased from University Wafer. Deionized (DI) water was prepared using a Milli-Q Gradient water purification system (Millipore) with a resistivity ρ ~ 18.2 MΩ cm at 25°C. Gold, Au, was purchased as 99.999% purity pellets from Kurt J. Lesker. Dulbecco’s Phosphate Buffered Saline (PBS, 1x, without calcium and magnesium) was purchased from Lonza. Sylgard 184 Silicone Elastomer Kit was purchased from Dow Corning Corporation. SU-8 2050 and SU-8 Developer were purchased from MicroChem. Photomasks were purchased from Front Range Photomask. All reagents were used as received.
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2

Phenolic Compounds Extraction and Identification in Cherries

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All
the chemicals used
in the extraction and identification of phenolic compounds in cherries
were of analytical grade. Gallic acid, quercetin, catechin, l-ascorbic acid, and EDTA standards for antioxidant assays were purchased
from Sigma-Aldrich (Castle Hill, NSW, Australia). The chemicals used
in antioxidant assays were Folin–Ciocalteu’s phenol
reagent, aluminum chloride hexahydrate, vanillin, sodium phosphate,
hydrated sodium acetate, trichloroacetic acid, hydrochloric acid,
2,2′-diphenyl-1-picrylhydrazl (DPPH), 2,4,6-tripyridyl-s-triazine (TPTZ), 2–2′-azino-bis(3-ethylbenz-thiazoline-6-sulphonate)
(ABTS), potassium ferrocyanide(III), iron(II) sulfate heptahydrate,
ferrozine, ferric(III) chloride anhydrous, 3-hydrobenzoic acid, and
ammonium molybdate. Anhydrous sodium carbonate was purchased from
Chem-Supply Pvt. Ltd. (Melbourne, VIC, Australia), and sodium acetate
(hydrated) was purchased from Ajax Finecham (Melbourne, VIC, Australia).
Methanol, ethanol, acetic acid, glacial acetic acid, acetonitrile,
and hydrochloric acid were purchased from Thermo Fisher Scientific
Inc. (Scoresby, VIC, Australia). Sulfuric acid (98%) was procured
from RCI Labscan Ltd. (Bangkok, Thailand). The deionized water used
in the study was prepared by the Millipore Milli-Q Gradient Water
Purification System (Darmstadt, Germany).
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3

Analytical Extraction and Characterization of Vigna Pulses

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Analytical grade chemicals were utilized for extraction and characterization of Vigna pulses. The 98% sulfuric acid was gained from RCI Labscan Ltd., Bangkok, Thailand. The sodium carbonate anhydrous was acquired from Chem-Supply Pty Ltd., Adelaide, SA, Australia. The majority of analytical grade chemicals, along with standards utilized in this research were obtained from Sigma-Aldrich (Castle Hill, NSW, Australia), containing quercetin, gallic acid, catechin, vanillin, and 6-hydroxy-2,5,7,8-tetramethylchrom an-2-carboxylic acid (Trolox), 2,2′-diphenyl-1-picrylhydrazyl (DPPH), Folin–Ciocalteu reagent, 2,4,6-tripyridyl-s-triazine (TPTZ), hydrated sodium acetate, hexahydrate aluminum chloride, ferric chloride (Fe (III) Cl3·6H2O), along with potassium chloride, sodium chloride, potassium dihydrogen phosphate, sodium hydrogen carbonate, magnesium dichloride, sodium hydroxide pellets, ammonium carbonate, calcium chloride, hydrogen chloride, pepsin, as well as bile salts. The in vitro digestive enzymes, such as α-amylase and pancreatin, were bought from US Biological (Assay Matrix Pty Ltd., Clyde, VIC, Australia). Milli-Q water utilized in this research was from Millipore Milli-Q Gradient Water Purification System (Darmstadt, Germany).
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