Purified recombinant ALK or ALK LF655del proteins were incubated with recombinant human MMP-9 (Biolegend) in reaction buffer (50 mM Tris-HCl buffer, pH 7.4, 150 mM NaCl) containing 0.2 mM p-aminophenylmercuric acetate (Calbiochem) for 3 hr. at 37°C. The samples were then denatured using NuPAGE LDS sample buffer and NuPAGE sample reducing reagent (Invitrogen), loaded onto SDS-PAGE (4%–12% Bio-Tris) gels and WB performed as described above.
Recombinant human mmp 9
Manufactured by BioLegend
Recombinant human MMP-9 is a protein produced using recombinant DNA technology. MMP-9, also known as matrix metalloproteinase-9, is an enzyme that plays a role in the breakdown and remodeling of extracellular matrix components.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using recombinant human mmp 9
1
ALK Protein Cleavage by MMP-9
2
ALK Protein Cleavage by MMP-9
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Purified recombinant ALK or ALK LF655del proteins were incubated with recombinant human MMP-9 (Biolegend) in reaction buffer (50 mM Tris-HCl buffer, pH 7.4, 150 mM NaCl) containing 0.2 mM p-aminophenylmercuric acetate (Calbiochem) for 3 hr. at 37°C. The samples were then denatured using NuPAGE LDS sample buffer and NuPAGE sample reducing reagent (Invitrogen), loaded onto SDS-PAGE (4%–12% Bio-Tris) gels and WB performed as described above.
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