Uv 2800

Manufactured by Hitachi

Sourced in Japan

The Hitachi UV-2800 is a UV-Vis spectrophotometer designed for accurate and reliable absorbance measurements across a wide range of wavelengths. It features a high-performance monochromator and a dual-beam optical system to provide precise and stable readings.

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Lab products found in correlation

Hippuryl l histidyl l leucine hhl, by Merck Group (1 mentions) Leu pna, by Merck Group (1 mentions)

Spelling variants of this product

U-2800 UV–Vis spectrophotometer (6 citations) U-2800 Double Beam UV/VIS Spectrophotometer (2 citations)

5 protocols using uv 2800

Hippuryl-L-Histidyl-L-Leucine ACE Inhibitor Assay

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Hippuryl-L-Histidyl-L-Leucine (HHL, Sigma-Aldrich, St. Louis, MO, USA) was used
as an enzyme-substrate. A total of 50 μL of the substrate (50 mM HHL in
0.1 M sodium borate buffer containing 0.3 M NaCl at pH 8.3) was added into a 50
μL sample and incubated at 37°C for 5 min. To initiate the
reaction, 50 μL of 0.1 U/mL ACE (Rabbit lung, Sigma-Aldrich) solution was
added, and the mixture was incubated at 37°C for 5 min. The reaction was
stopped by adding 250 μL 1 M HCl. The resulted hippuric acid (HA) was
extracted with 1.5 mL ethyl acetate and centrifuged at 2,000×g for 5 min.
An aliquot (0.8 mL) of the ethyl acetate layer was transferred to a clean tube
and evaporated at 85°C for 60 min. Distilled water (4 mL) was then added
to dissolve the HA in the tube, and the amount of HA formed was measured by
measuring the optical density at 228 nm (UV-2800, Hitachi, Tokyo, Japan). The
extent of inhibition was calculated as 100% [(B – A) / B] where A
is the optical density in the presence of ACE and ACEI components, and B is the
optical density without the ACEI component.

Rubak Y.T., Nuraida L., Iswantini D, & Prangdimurti E. (2022). Angiotensin-I-Converting Enzyme Inhibitory Peptides in Goat Milk Fermented by Lactic Acid Bacteria Isolated from Fermented Food and Breast Milk. Food Science of Animal Resources, 42(1), 46-60.

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Quantification of CPT-loaded Micelles by UV-Vis

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CPT loading level was measured by UV-vis spectrophotometer (UV-2800, Hitachi, Japan).^{37 (link)} Lyophilized CPT-loaded micelles were re-dissolved in DMF and the concentration was calculated according to a standard curve of pure CPT/DMSO solution. Drug loading content (DLC) and drug loading efficiency (DLE) were determined according to the following formulas:where the weight of drug in feed represents the total amount of CPT added into the system.
The standard curve of CPT concentration-UV absorption: CPT dissolved into DMF to prepare the solution with various predetermined concentrations, the standard curve was obtained by plotted the absorbance of CPT at λ = 365 nm against various predetermined concentrations (R = 0.99982).

Sun J., Wang Z., Cao A, & Sheng R. (2019). Synthesis of crosslinkable diblock terpolymers PDPA-b-P(NMS-co-OEG) and preparation of shell-crosslinked pH/redox-dual responsive micelles as smart nanomaterials. RSC Advances, 9(59), 34535-34546.

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In Vitro Determination of ACE-I Activity

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The determination of ACE-I activity was performed in vitro based on the method of Chusman and Cheung [24 (link)]. Hippuryl-L-Histidyl-L-Leucine (HHL, Sigma, USA) was used as the enzyme-substrate. A 50 µL of the substrate (50 mM HHL in 0.1 M sodium borate buffer containing 0.3 M NaCl at pH 8.3) was added into 50 µL sample and incubated at 37°C for 5 min. To initiate the reaction, 50 µL of 0.1 U/mL ACE (Sigma, USA) solution was added, and the mixture was incubated at 37°C for 5 min. The reaction was stopped by adding 250 µL 1 M HCl 1 M. The resulted in hippuric acid (HA) was extracted with 1.5 mL ethyl acetate and centrifuged at 2000× g for 5 min. An aliquot (0.8 mL) of the ethyl acetate layer was transferred to a clean tube and evaporated at 85°C for 60 min. Distilled water (4 mL) was then added to dissolve the HA in the tube, and the amount of HA formed was measured by measuring optical density at 228 nm (UV-2800, Hitachi, JPN) The extent of inhibition was calculated as 100% ([B-A]/B) where A is the optical density in the presence of ACE and ACE-I component, B is the optical density without ACE-I component.

Rubak Y.T., Nuraida L., Iswantini D, & Prangdimurti E. (2020). Angiotensin-I-converting enzyme inhibitory peptides in milk fermented by indigenous lactic acid bacteria. Veterinary World, 13(2), 345-353.

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Quantifying Drug Loading in Nanoparticles

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The drug-loading content was measured by an ultraviolet-visible (UV-Vis) spectrophotometry (UV-2800, Hitachi, Tokyo, Japan). The Dr.S/RAD001 nanoparticles were dissolved in 20 mL of water and absorbance measured at λ = 279 nm. A calibration curve was established under identical conditions. The drug loading content was calculated using the following formula:

Yuan Y., Jin P., Wang Y., Zhao X., Hu Q., Wu W., Huang J, & Zhang N. (2020). A dendritic, redox-responsive, supramolecular (Dr.S) system for lysis-triggered delivery for drug-resistant renal cancer. RSC Advances, 10(62), 37826-37833.

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Leu-pNA Assay for LAP Activity

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LAP activity was determined using Leu-pNA (Sigma-Aldrich Japan, Tokyo, Japan) as a substrate. The sample (0.1 mL) and 0.1 mL of the substrate (32 mM) were added to 0.8 mL of a 100 mM Tris-HCl (pH 8.0) and 1 mM CaCl 2 solution. The amount of p-nitroaniline released was determined by measuring the absorbance at 405 nm using a spectrophotometer (UV2800, Hitachi Ltd.). The initial velocity was determined from the linear region of the optical density pro le (ε405nm= 10,600 M -1 cm -1 ) (Arima et al. 2006) .

Uraji M., Yang L, & Hatanaka T. (2022). Improvement in hyperexpression with a combination of truncated scmp promoter and Streptomyces lividans. Bioscience, biotechnology, and biochemistry, 86(8).

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