Sting inhibitor h151

HEK293T and U87 cells were maintained in DMEM (Gibco) supplemented with 10% foetal bovine serum (FBS, LabTech) and 100 U/ml penicillin plus 100 μg/ml streptomycin (Pen/Strep; Gibco). THP‐1 cells were maintained in RPMI (Gibco) supplemented with 10% FBS and Pen/Strep. THP‐1‐IFIT‐1 cells that had been modified to express Gaussia luciferase under the control of the IFIT‐1 promoter were described previously (Mankan et al, 2014). THP‐1 Dual Control and cGAS^−/− cells were obtained from Invivogen. Lopinavir (LPV), darunavir (DRV), nevirapine (NVP) and raltegravir were obtained from AIDS reagents. STING inhibitor H151 was obtained from Invivogen. JAK inhibitor ruxolitinib was obtained from CELL guidance systems. PF‐74 was obtained from Sigma. Lipopolysaccharide, IFNβ and poly I:C were obtained from PeproTech. Sendai virus was obtained from Charles River Laboratories. Herring testis DNA was obtained from Sigma. cGAMP was obtained from Invivogen. Anti‐IFNα/β receptor and control IgG2A antibodies were obtained from PBL Interferon Source and R&D systems, respectively. For stimulation of cells by transfection, transfection mixes were prepared using lipofectamine 2000 according to the manufacturer's instructions (Invitrogen).

HEK293T, FRhK and U87 cells were maintained in DMEM (Gibco) supplemented with 10% foetal bovine serum (FBS, Labtech) and 100 U/ml penicillin plus 100 µg/ml streptomycin (Pen/Strep; Gibco). THP-1 cells were maintained in RPMI (Gibco) supplemented with 10% FBS and Pen/Strep. THP-1-IFIT-1 cells that had been modified to express Gaussia luciferase under the control of the IFIT-1 promoter [33 (link)] and versions lacking MAVS or STING [34 ] were described previously. THP-1 cells stably depleted for SAMHD1 were also previously described [26 (link)]. THP-1 Dual Control and cGAS-/- cells were obtained from Invivogen. Nevirapine and raltegravir were obtained from AIDS reagents. STING inhibitor H151 and cGAS inhibitor RU.521 were obtained from Invivogen. JAK inhibitor ruxolitinib was obtained from CELL guidance systems. Lipopolysaccharide and IFNβ were obtained from Peprotech. Herring-testis DNA was obtained from Sigma. cGAMP and poly I: C were obtained from Invivogen. For stimulation of cells by transfection, transfection mixes were prepared using lipofectamine 2000 according to the manufacturer’s instructions (Invitrogen).