Phix174 hinfi digest dna ladder

Manufactured by Promega

The PhiX174 HinfI digest DNA ladder is a molecular weight marker used to determine the size of DNA fragments in gel electrophoresis. It contains DNA fragments of known sizes that can be used as a reference to estimate the size of unknown DNA samples.

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Lab products found in correlation

Deoxyribonucleotides, by Roche (3 mentions) Dna oligonucleotide, by Integrated DNA Technologies (3 mentions) G 25 spin columns, by Harvard Apparatus (3 mentions) T4 polynucleotide kinase pnk, by New England Biolabs (2 mentions) Radiolabeled compounds, by PerkinElmer (2 mentions) Terminal transferase tdt, by New England Biolabs (1 mentions) Protran ba 85, by Cytiva (1 mentions) Deae filtermat, by PerkinElmer (1 mentions) Rapid dna ligation kit, by Promega (1 mentions) Plasmid dna miniprep kit, by Qiagen (1 mentions) Calf intestinal alkaline phosphatase cip, by New England Biolabs (1 mentions) T3 rna polymerase, by New England Biolabs (1 mentions) Mulv rt, by New England Biolabs (1 mentions) Rneasy rna purification, by Qiagen (1 mentions) Rnasin rnase inhibitor, by Promega (1 mentions)

3 protocols using phix174 hinfi digest dna ladder

Enzymatic Manipulation of Nucleic Acids

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Exonuclease-free Klenow fragment of Escherichia coli DNA polymerase I, terminal transferase (TdT), and T4 polynucleotide kinase (PNK) were from New England Biolabs. DNase (deoxyribonuclease)-free RNase (ribonuclease), ribonucleotides, and deoxyribonucleotides were obtained from Roche. RNase-free DNase I was from United States Biochemical. The phiX174 Hinf I digest DNA ladder was from Promega. Radiolabeled compounds were from PerkinElmer. DNA oligonucleotides were from Integrated DNA Technologies. G-25 spin columns were from Harvard Apparatus. AZTTP was obtained from PerkinElmer, and ddCTP and ddGTP were from United States Biologicals. Nevirapine (NVP), rilpivirine (RPV), and efavirenz (EFV) were obtained from the National Institutes of Health AIDS Research and Reference Reagent Program. All other chemicals were obtained from Fisher Scientific, VWR, or Sigma.

Achuthan V., Singh K, & DeStefano J.J. (2016). Physiological Mg2+ Conditions Significantly Alter the Inhibition of HIV-1 and HIV-2 Reverse Transcriptases by Nucleoside and Non-Nucleoside Inhibitors in Vitro. Biochemistry, 56(1), 33-46.

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Radiolabeled Ligand Binding Assay

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T4 polynucleotide kinase (PNK) was from New England Biolabs. Deoxyribonucleotides were obtained from Roche. The phiX174 HinfI digest DNA ladder was from Promega. DNA oligonucleotides were from Integrated DNA Technologies. G-25 spin columns were from Harvard Apparatus. Centrifugal filters (Amicon Ultra −0.5 mL 30 000 nominal molecular weight cutoff (Ultracel-30K)) were from Merck Millipore Ltd. NNRTIs, nevirapine (NVP), rilpivirine (RPV), and efavirenz (EFV) were obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH. After resuspension in DMSO, NNRTIs were aliquoted and stored at −80 °C. Radiolabeled EFV (¹⁴C, >50 mCi/mmol) was from ViTrax. Radiolabeled ATP ([γ-³²P] 3000 Ci/mmol, 10 mCi/mL), dTTP ([α-³²P] 3000 Ci/mmol, 10 mCi/mL), dTTP ([methyl-³H] 70–90 Ci (2.59–3.33 TBq)/mmole), and glass fiber DEAE Filtermats were from PerkinElmer. Nitrocellulose filters disks (Protran BA 85, 0.45 μm pore size and 25 mm diameter) were from Whatman. All other chemicals were obtained from Fisher Scientific, VWR, or Sigma.

, & DeStefano J.J. (2019). Non-nucleoside Reverse Transcriptase Inhibitors Inhibit Reverse Transcriptase through a Mutually Exclusive Interaction with Divalent Cation-dNTP Complexes. Biochemistry, 58(16), 2176-2187.

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Biochemical Reagents for Molecular Biology Experiments

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Calf intestinal alkaline phosphatase (CIP), T3 RNA polymerase, “High Fidelity” (PvuII and EcoRI) and other restriction enzymes, T4 polynt kinase (PNK), and MuLV RT were from New England Biolabs. DNase (deoxyribonuclease)-free RNase (ribonuclease), ribonucleotides, and deoxyribonucleotides were obtained from Roche. RNase free-DNase I was from United States Biochemical. Rapid DNA ligation kit, RNasin (RNase inhibitor), and the phiX174 HinfI digest DNA ladder was from Promega. Radiolabeled compounds were from PerkinElmer. Pfu DNA polymerase was from Stratagene. DNA oligonucleotides were from Integrated DNA Technologies. G-25 spin columns were from Harvard Apparatus. RNeasy RNA purification and the Plasmid DNA Miniprep kits were from Qiagen. X-gal was from Denville Scientific, Inc. IPTG and media were from Gibco, Life Technologies. All other chemicals were obtained from Fisher Scientific, VWR, or Sigma. HIV RT (from HXB2 strain) was prepared as described [64 (link)]. The HIV RT clone was a generous gift from Dr. Michael Parniak (University of Pittsburgh). This enzyme is a non-tagged heterodimer consisting of equal proportions of p66 and p51 subunits. Aliquots of HIV RT were stored frozen at −80°C and fresh aliquots were used for each experiment.

Achuthan V, & DeStefano J.J. (2015). Alternative divalent cations (Zn2+, Co2+, and Mn2+) are not mutagenic at conditions optimal for HIV-1 reverse transcriptase activity. BMC Biochemistry, 16, 12.

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