Bond max automated slide stainer

Manufactured by Leica

The BOND-MAX is an automated slide stainer system designed for clinical laboratories. It automates the process of staining and preparation of tissue samples on slides, allowing for efficient and consistent processing.

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Lab products found in correlation

Pre treatment solution, by Agilent Technologies (1 mentions) Bond er solution 1, by Leica (1 mentions) Icc50w camera, by Leica (1 mentions) Bond wash solution, by Leica (1 mentions) Bond polymer refine red kit, by Leica (1 mentions) Bond dewax solution, by Leica (1 mentions) Bond max, by Leica (1 mentions)

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Bond Max (596 citations) Leica BOND-MAX stainer (24 citations) Automated stainer (4 citations) Automated slide stainer (3 citations) Bond-Max slide stainer (2 citations) Leica stainer (2 citations)

2 protocols using bond max automated slide stainer

Fluorescence In-Situ Hybridization Protocol for MYB and EBV Analysis

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Fluorescence in-situ hybridization (FISH) was performed as described previously^{43 (link)} using the MYB Dual Color Break Apart Probe (Zytovision). In brief, 4 µm sections were deparaffinized dehydrated and incubated in pretreatment solution (Dako, Denmark) at 95–99 °C for 10 min. Following immersion in pepsin solution for 3–6 min at 37 °C, slides were washed, dehydrated and air dried. DNA probes were applied and the sections were sealed and denaturalized in humidified atmosphere at 82 °C for 5 min. Sections hybridized at 45 °C overnight. After washing, slides were counterstained with 4′,6-diamidino-2-phenylindole (DAPI).
Silver-enhanced in-situ hybridization for EBV analysis was done using the BOND Epstein-Barr virus-encoded small RNA (EBER) Probe (Leica) on the BOND-MAX automated slide stainer (Leica).

Jurmeister P., Glöß S., Roller R., Leitheiser M., Schmid S., Mochmann L.H., Payá Capilla E., Fritz R., Dittmayer C., Friedrich C., Thieme A., Keyl P., Jarosch A., Schallenberg S., Bläker H., Hoffmann I., Vollbrecht C., Lehmann A., Hummel M., Heim D., Haji M., Harter P., Englert B., Frank S., Hench J., Paulus W., Hasselblatt M., Hartmann W., Dohmen H., Keber U., Jank P., Denkert C., Stadelmann C., Bremmer F., Richter A., Wefers A., Ribbat-Idel J., Perner S., Idel C., Chiariotti L., Della Monica R., Marinelli A., Schüller U., Bockmayr M., Liu J., Lund V.J., Forster M., Lechner M., Lorenzo-Guerra S.L., Hermsen M., Johann P.D., Agaimy A., Seegerer P., Koch A., Heppner F., Pfister S.M., Jones D.T., Sill M., von Deimling A., Snuderl M., Müller K.R., Forgó E., Howitt B.E., Mertins P., Klauschen F, & Capper D. (2022). DNA methylation-based classification of sinonasal tumors. Nature Communications, 13, 7148.

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CDKN2A/p16 Immunohistochemistry for FFPE Tumor Tissues

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CDKN2A/p16 immunohistochemistry (IHC), on FFPE tumor tissues, was performed with a BOND-MAX automated staining platform (Leica Biosystems, Buccinasco, Italy), according to standard procedure. The specimens were sectioned at a thickness of 3 μm and stained on glass slides baked at 60°C for 30 minutes. Deparaffinization, rehydration and antigen retrieval were performed by Bond Dewax Solution, Bond Wash Solution, ethanol and Bond ER Solution 1 (prediluted; pH 6.0) antigen retrieval solution (Leica), performed on the BOND-MAX automated slide stainer (Leica) for 30 minutes at 95°C. The ready-to-use primary CDKN2A/p16 primary anti-human antibody (6H12; Leica), was incubated for 20 minutes at room temperature, followed by visualization with the Bond Polymer Refine Red Kit (Leica). The specimens were counter-stained with hematoxylin. Slide fixation was performed with mounting medium and observation under optical microscope (Leica DM750) equipped with Leica ICC50W camera (Leica).

Merlini A., Centomo M.L., Ferrero G., Chiabotto G., Miglio U., Berrino E., Giordano G., Brusco S., Pisacane A., Maldi E., Sarotto I., Capozzi F., Lano C., Isella C., Crisafulli G., Aglietta M., Dei Tos A.P., Sbaraglia M., Sangiolo D., D’Ambrosio L., Bardelli A., Pignochino Y, & Grignani G. (2022). DNA damage response and repair genes in advanced bone and soft tissue sarcomas: An 8-gene signature as a candidate predictive biomarker of response to trabectedin and olaparib combination. Frontiers in Oncology, 12, 844250.

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