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Xradia 810 ultra confocal laser scanning microscope

Manufactured by Zeiss
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The Xradia 810 Ultra Confocal Laser Scanning Microscope is a high-resolution imaging system designed for non-destructive 3D visualization and analysis of samples. It utilizes a confocal laser scanning approach to capture detailed images with high spatial resolution.

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Lab products found in correlation

Zen 2012, by Zeiss (2 mentions) Alexa fluor 488 affinipure donkey anti rabbit igg, by Jackson ImmunoResearch (2 mentions) Rhodamine phalloidin, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 affinipure donkey anti mouse igg, by Jackson ImmunoResearch (2 mentions)

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Confocal microscope (860 citations) Confocal laser scanning microscope (782 citations) Laser confocal microscope (170 citations) Laser scanning microscope (67 citations) Xradia Ultra 810 (8 citations) 810 confocal microscope (5 citations) Confocal 810 (2 citations)

2 protocols using xradia 810 ultra confocal laser scanning microscope

1

Immunocytochemical Analysis of Key Signaling Proteins

Cited 1 time
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The expression and cellular location of YAP1, IRF3, IRF9, NFKB1, and STAT1 were analyzed by fluorescent immunocytochemistry as described previously (Lv et al., 2017 (link)). Cells were grown on coverslips in a 24-well plate. After treatment, cells were fixed with 4% formaldehyde (in 1X PBS) at 4°C for 20 minutes, rinsed with PBST for 3 times X 5 minutes, blocked cells with normal donkey serum (10%) for 1 hour before incubating with primary antibody (~200x) at 4°C for 16 hours. Antigen was visualized with fluorochrome-conjugated secondary antibodies. Fluorescence-conjugated secondary antibodies for immunofluorescent analyses, including Alexa Fluor® 488 AffiniPure Donkey Anti-Rabbit IgG (#711-545-152) and Alexa Fluor® 488 AffiniPure Donkey Anti-Mouse IgG (#715-545-150), were from the Jackson Immunoresearch Laboratories Inc. (West Grove, PA); Rhodamine Phalloidin (#R415) for visualizing actin was from Thermo Fisher Scientific (Rockford, IL). Nuclei were stained with DAPI. Images were captured using a ZEISS Xradia 810 Ultra Confocal Laser Scanning Microscope and analyzed with Zeiss Zen 2012 software (Carl Zeiss Microscopy, LLC, Thornwood, NY).
He C., Lv X., Huang C., Angeletti P.C., Hua G., Dong J., Zhou J., Wang Z., Ma B., Chen X., Lambert P.F., Rueda B.R., Davis J.S, & Wang C. (2019). A Human Papillomavirus-Independent Cervical Cancer Animal Model Reveals Unconventional Mechanisms of Cervical Carcinogenesis. Cell reports, 26(10), 2636-2650.e5.
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2

Immunocytochemical Analysis of Key Signaling Proteins

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The expression and cellular location of YAP1, IRF3, IRF9, NFKB1, and STAT1 were analyzed by fluorescent immunocytochemistry as described previously (Lv et al., 2017 (link)). Cells were grown on coverslips in a 24-well plate. After treatment, cells were fixed with 4% formaldehyde (in 1X PBS) at 4°C for 20 minutes, rinsed with PBST for 3 times X 5 minutes, blocked cells with normal donkey serum (10%) for 1 hour before incubating with primary antibody (~200x) at 4°C for 16 hours. Antigen was visualized with fluorochrome-conjugated secondary antibodies. Fluorescence-conjugated secondary antibodies for immunofluorescent analyses, including Alexa Fluor® 488 AffiniPure Donkey Anti-Rabbit IgG (#711-545-152) and Alexa Fluor® 488 AffiniPure Donkey Anti-Mouse IgG (#715-545-150), were from the Jackson Immunoresearch Laboratories Inc. (West Grove, PA); Rhodamine Phalloidin (#R415) for visualizing actin was from Thermo Fisher Scientific (Rockford, IL). Nuclei were stained with DAPI. Images were captured using a ZEISS Xradia 810 Ultra Confocal Laser Scanning Microscope and analyzed with Zeiss Zen 2012 software (Carl Zeiss Microscopy, LLC, Thornwood, NY).
He C., Lv X., Huang C., Angeletti P.C., Hua G., Dong J., Zhou J., Wang Z., Ma B., Chen X., Lambert P.F., Rueda B.R., Davis J.S, & Wang C. (2019). A Human Papillomavirus-Independent Cervical Cancer Animal Model Reveals Unconventional Mechanisms of Cervical Carcinogenesis. Cell reports, 26(10), 2636-2650.e5.
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