Sk rst cells

Porcine parvovirus strain NADL-2 was kindly provided by Dr Albert Bosch (Department of Genetics, Microbiology and Statistics School of Biology, University of Barcelona, Spain). It was propagated in SK-RST cells (ATCC CRL-2842), grown in SGM supplemented with 5% FBS. One mL of virus stock and 9 mL of MEM-E supplemented with 1% pyruvate (Merck KGaA, Darmstadt, Germany) were added to a conical tube with 16 x 10⁶ SK-6 cells and shaken for 30 minutes at 104 rpm and 37°C. After that time, the contents of the tube were transferred to a 175 cm² flask, in which 40 mL of MEM-E supplemented with 1% pyruvate were added. This procedure was repeated until obtaining 811 mL of viral suspension with a titer of 10^6.91 TCID_50% /mL that was used to inoculate 24 L of bovine plasma achieving a final viral titer of 10^5.44 TCID₅₀ /mL.
Genomic data and virion size of each virus used in this study are displayed in Table 1.

Porcine parvovirus strain NADL-2 was kindly provided by Dr Albert Bosch (Department of Genetics, Microbiology and Statistics School of Biology, University of Barcelona, Spain). It was propagated in SK-RST cells (ATCC CRL-2842), grown in SGM supplemented with 5% FBS. One mL of virus stock and 9 mL of MEM-E supplemented with 1% pyruvate (Merck KGaA, Darmstadt, Germany) were added to a conical tube with 16 x 10⁶ SK-6 cells and shaken for 30 minutes at 104 rpm and 37ºC. After that time, the contents of the tube were transferred to a 175 cm² flask, in which 40 mL of MEM-E supplemented with 1% pyruvate were added. Inoculated flasks were incubated for four days at 37ºC until CPE was observed. A viral suspension was obtained and titrated in triplicate, obtaining a final viral solution of 10^6.6±0.2 TCID₅₀ /mL.