For detecting chemo- and cytokines in the supernatant of differentiated, polarized, or activated MdM and MdDC, the cytometric bead array (BD Bioscience, Heidelberg, Germany) was used for TNF-α, IL-1β, IL-6, IL-10, CCL2, CXCL8, and CXCL10, while ELISAs were used to detect IL-23 (Thermo Fisher, Waltham, MA, USA), CCL17 (BioLegend, Fell, Germany), CCL18 (Boster Biological Technology, Pleasanton, CA, USA), and PGE2 (Enzo, Lörrach, Germany). cytometric bead array was performed according to the manufacturer’s protocol. In a deviation from protocol, only half of the capture and detection beads and 25 μl of the supernatant were used. ELISAs were performed strictly according to the manufacturer’s protocol. For the ELISAs, 100 μl of the supernatant was used.
Ccl17
Manufactured by BioLegend
Sourced in United States
CCL17 is a chemokine that plays a role in the recruitment and activation of T cells. It is involved in the immune response and inflammation processes.
Automatically generated - may contain errors
3 protocols using ccl17
1
Cytokine Profiling of Polarized MdM and MdDC
2
Transwell assay for ILC2 migration
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Cell migration was investigated using 5-µm transwell inserts for 24-well cell culture plates (Sarstedt). Briefly, 2 × 105 in vitro–expanded ILC2s in ILC2 medium were placed in the upper compartment. The well below was filled with ILC2 medium containing the recombinant chemokine CCL1, CCL8, CCL17, or CCL22 (all from BioLegend) at the indicated concentration or 5 µM of the activating CCR8 agonist ZK756326 (Selleckchem). After incubation for 2.5 h in a 37°C incubator, the migrated cells in the lower compartment were counted using a Neubauer chamber. The chemotaxis index was defined as the ratio of migrated cells to the respective stimulus and migrated cells without chemokines.
3
Quantification of Chemokine Levels
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