Phenotypic features were described from colonies growing on malt extract Agar (MEA). Malt extract 20 g/L, peptone 1 g/L, and D-glucose 20 g/L, Agar 20 g/L (HiMedia, Mumbai, India), OA (Sigma–Aldrich, St. Louis, MO, USA), and PCA (HiMedia, Mumbai, India) at 25 °C. Colony colours were assessed according to The Royal Horticultural Society London [16 ]. Micromorphological descriptions and measurements for 30 replicates of sexual structures and relevant features were carried out in lactic acid 90%. Photomicrographs were taken with a Keyence VHX-970F microscope (Neu-Isenburg, Germany) and a Nikon eclipse Ni compound microscope, using a DS-Fi3 (Nikon, Tokyo, Japan) and NIS-Elements imaging software v. 5.20.
D glucose
Manufactured by HiMedia
Sourced in India
D-glucose is a monosaccharide, the simplest form of carbohydrates. It serves as a primary source of energy for many organisms and plays a crucial role in various metabolic processes.
Automatically generated - may contain errors
Lab products found in correlation
Eclipse ni compound microscope, by Nikon (1 mentions)
Ds fi3, by Nikon (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Folic acid, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by HiMedia (1 mentions)
Dichlorofluorescein diacetate dcfh da, by Merck Group (1 mentions)
Penicillin streptomycin solution, by HiMedia (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Mcf 7, by National Centre for Cell Science (1 mentions)
Fetal calf serum (fcs), by HiMedia (1 mentions)
5 bromo 4 chloro 3 indolyl β d galacto pyranoside x gal, by Thermo Fisher Scientific (1 mentions)
Spectinomycin, by Merck Group (1 mentions)
M17 medium, by Merck Group (1 mentions)
Erythromycin, by Serva Electrophoresis (1 mentions)
Luria bertani broth, by HiMedia (1 mentions)
Ascorbic acid, by HiMedia (1 mentions)
Creatinine, by HiMedia (1 mentions)
L cysteine, by HiMedia (1 mentions)
Glycine, by HiMedia (1 mentions)
6 protocols using d glucose
1
Detailed Fungal Characterization Protocol
2
Cellular Assays for Folic Acid Derivatives
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Folic acid and 5-MTHF disodium salt were purchased from Sigma (Sigma–Aldrich, Mumbai, India). 10-FTHF was from Schircks Laboratories (Jona, Switzerland). Dulbecco’s modified Eagle medium (DMEM), minimum essential medium Eagle (MEM), phosphate-buffered saline (PBS), penicillin–streptomycin solution, foetal bovine serum (FBS), trypsin–EDTA solution, bovine serum albumin (BSA), and d -glucose were obtained from HiMedia (Mumbai, India). Dichlorofluorescein diacetate (DCFH-DA) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were procured from Sigma (Sigma–Aldrich, India). Antibodies were procured from Cloud-Clone (Cloud-Clone Corporation, Houston, TX).
3
Cell Line Characterization for Uptake and Toxicity
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Cell line -For in vitro cellular uptake and cytotoxicity, human cervical cancer cell line, MCF-7, and MDA-MB 231 were used. HeLa cells were obtained from Roswell Park Memorial Institute and which are cultured in RPMI-1640 and DMEM and supplemented with 4.5 g/L D-glucose, 1 mM sodium pyruvate, 1.5 g/L sodium bicarbonate, and 10% fetal calf serum (HiMedia Lab, Mumbai). The cells were maintained at 37°C and 5% CO 2 in a CO 2 incubator. MCF-7 and MDA-MB 231 were obtained from the National Centre for Cell Sciences, Pune and are grown in L-15 (Invitrogen, USA) supplemented with 10% fetal bovine serum (Invitrogen, USA), 100 units of penicillin/streptomycin (Invitrogen, USA) at 37°C and 5% CO 2 in a CO 2 incubator.
4
Testosterone Propionate Assay Protocol
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Testosterone propionate (TP), olive oil, and D glucose were obtained from HiMedia Laboratories Pvt. Ltd., Mumbai, India. Glucose oxidase kit was procured from Erba Mannheim (Transasia), Mumbai, India. Testosterone kit was purchased from Diagnostics Biochem Canada (DBC), Ontario, Canada.
5
Antibiotic selection and MIC determination for lactococci
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All strains and derivatives used in this study are listed in Table 1. Lactococcal strains were grown in M17 medium (Merck GmbH, Darmstadt, Germany) supplemented with D-glucose (HiMedia Laboratories, Mumbai, India) (0.5 % w/v) (GM17) at 30 • C. Escherichia coli DH5α was grown aerobically in Luria-Bertani (LB) broth (HiMedia Laboratories, Mumbai, India) at 37 • C. Solid medium was made by adding 1.75 % (w/ v) agar (Torlak, Belgrade, Serbia), to the liquid media. Antibiotics were used at the following concentrations: Erythromycin (SERVA Electrophoresis GmbH, Heidelberg, Germany) was used at 300 μg/mL for E. coli and 10 μg/mL for lactococci for selection and maintaining of transformants. Spectinomycin (Merck KGaA, Darmstadt, Germany) was used at 250 and 500 μg/mL for maintaining of invertants and 50, 100, 150, 200, 250, 300, 350, 400 and 500 μg/mL for determination of MIC values for Spectinomycin of transformants in lactococci. For blue/white colour screening, 5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside (X-Gal) (Fermentas, Vilnius, Lithuania) was added to LB medium plates of colonies with cloned fragments at a final concentration of 40 μg/mL.
6
Biosensing of Biomolecules Using TE-Derived Nanoparticles
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TE was collected from the botanical garden of the BHU campus, Varanasi, India. Other chemical reagents were procured from Sigma Aldrich. For the dilution of the reagents required during synthesis, DI water was used. All the sensing measurements were done with DI water. The metal salts l -cysteine, d -glucose, glycine, creatinine, ascorbic acid, uric acid, and Tris HCl buffer were purchased from HiMedia, Merck, and Sigma Aldrich. Different kinds of metal salts were also prepared in DI water for optical sensing. For the real sample sensing, HBS samples were collected from Prakash Pathology, Varanasi, India.
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