Cerulean

Manufactured by Merck Group

Cerulean is a state-of-the-art laboratory equipment designed for precise and efficient analysis. It employs advanced technology to deliver accurate and reliable data. The core function of Cerulean is to facilitate scientific research and investigations in a wide range of applications.

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Lab products found in correlation

Cerulein, by Merck Group (1 mentions) Control liposome, by Formumax Scientific (1 mentions) Celecoxib, by Selleck Chemicals (1 mentions) Clophosome, by Formumax Scientific (1 mentions) Guanosine, by Selleck Chemicals (1 mentions) 8 ohg, by Abcam (1 mentions) 4 hne, by Abcam (1 mentions) Liproxstatin 1, by Selleck Chemicals (1 mentions) L arginine, by Merck Group (1 mentions) C57bl 6 mice, by GemPharmatech (1 mentions) Fm1 43, by Thermo Fisher Scientific (1 mentions) Dmi6000b microscope, by Leica (1 mentions) Soybean trypsin inhibitor, by Merck Group (1 mentions) Collagenase p, by Roche (1 mentions) Glass bottom dishes, by MatTek (1 mentions)

4 protocols using cerulean

Induction of Acute Pancreatitis in Mice

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C57BL/6 mice (GemPharmatech) were housed in standard cages at 25°C with free access to water and a commercial rodent diet under pathogen‐free conditions. All experiments were performed in accordance with an experimental guideline approved by the Animal Care and Use Committee of the Second Hospital of Hebei Medical University.
Eight‐week‐old female C57BL/6 mice were intraperitoneally injected with 50 mg/kg cerulean (Sigma‐Aldrich) dissolved in saline to induce severe acute pancreatitis. Untreated mice were intraperitoneally injected with the same volumes of saline as the control. Pancreatic samples were collected from mice 12 h after a single injection of cerulean and cut into two parts. One was stored at −80°C and the other was fixed in 10% formaldehyde for histological analysis.
After pretreated with AT7519 (15 mg/kg) or dimethyl sulfoxide (DMSO; Sigma‐Aldrich), mice were intraperitoneally injected with 50 mg/kg cerulean. Then, mice were killed 12 h after cerulean injection.

Ma Y., Liu L., Li B., Wang W, & Zhao T. (2022). Cdk2 suppresses IL‐23 expression and the onset of severe acute pancreatitis. Immunity, Inflammation and Disease, 10(6), e631.

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Induction of Acute Pancreatitis in Mice

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Methods are described in detail in Lodestijn et al.²⁶ In brief, acute pancreatitis was induced in adult mice (60‐70 days old), which received intraperitoneal injections with cerulein (Sigma‐Aldrich, C9026) in PBS (50 μg/kg) every hour seven times a day for 2 consecutive days. On the days of cerulean treatment, Metamizol (Sigma‐Aldrich, 46232) in PBS (200 mg/kg) was three times orally administered as analgesia. Mice were sacrificed and pancreata were isolated either 9 days (pancreatitis) or 38 days (recovered postpancreatitis) after the last cerulein injection.

Dings M.P., Manoukian P., Waasdorp C., Quik J.S., Strijker M., Lodestijn S.C., van Neerven S.M., Moreno L.F., de Oliveira R.L., Bonsing B.A., Bruno M.J., Busch O.R., Doukas M., van Eijck C.H., Mohammad N.H., de Hingh I.H., Molenaar Q.I., Besselink M.G., Vermeulen L., Medema J.P., van Laarhoven H.W, & Bijlsma M.F. (2022). Serum levels of iCAF‐derived osteoglycin predict favorable outcome in pancreatic cancer. International Journal of Cancer, 152(3), 511-523.

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Oxidative Stress Pathway Analysis

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Cerulean (#C9026) and l-arginine (#A5131) were obtained from Sigma-Aldrich. 8-OHG (#ab145594) was obtained from Abcam. 4-HNE (#2083) was obtained from BioVision. Clophosome (#F70101C-N) and control liposomes (#F70101-N) were obtained from FormuMax Scientific. Liproxstatin-1 (#S7699), guanosine (#S2439), celecoxib (#S1261), and PGE2 (#S3003) were obtained from Selleck Chemicals.

Dai E., Han L., Liu J., Xie Y., Zeh H.J., Kang R., Bai L, & Tang D. (2020). Ferroptotic damage promotes pancreatic tumorigenesis through a TMEM173/STING-dependent DNA sensor pathway. Nature Communications, 11, 6339.

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Pancreatic Acini Exocytosis Imaging

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Isolation of dispersed pancreatic acini was performed by the enzymatic and mechanical dissociation technique using collagenase P (Roche) as described previously^{57 (link)}. Isolated acini were seeded in Waymouth’s media (Sigma-Aldrich) supplemented with 0.1% BSA and 0.2 mg/ml soybean trypsin inhibitor (Sigma-Aldrich) in glass bottom dishes (MatTek Corporation) coated with Cell-Tak tissue cell adhesive (BD Biosciences). The acinar cells were then incubated with 2 μmol/l FM1-43 (Invitrogen) at 37 °C and imaged on a DMI6000B microscope (Leica) as described^{58 (link)}. After obtaining stable basal fluorescence signals, cerulean (Sigma-Aldrich) was added to a final concentration of 1 nM to stimulate exocytosis of ZGs. Images were acquired every 1 min for 60 min.

Cyge B., Voronina V., Hoque M., Kim E.N., Hall J., Bailey-Lundberg J.M., Pazour G.J., Crawford H.C., Moon R.T., Li F.Q, & Takemaru K.I. (2021). Loss of the ciliary protein Chibby1 in mice leads to exocrine pancreatic degeneration and pancreatitis. Scientific Reports, 11, 17220.

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