Cell pellets were resolved by SDS-PAGE using 15% gels. Gels were either stained with Coomassie Brilliant Blue G-250 (Sigma_Aldrich, San Francisco, CA, USA) for confirming the bands of fusion proteins or processed for WB by electro-transferring the proteins onto 0.2 μm polyvinylidene difluoride (PVDF) membrane (Immobilon®-PSQ, Merck Millipore Ltd., Darmstadt, Germany). Membranes were then blocked with 5% (w/v) skimmed milk powder in tris-buffered saline with 0.1% tween 20 (TBST), incubated sequentially with anti-S serum (dilution, 1:4000 in TBST) and goat anti-rabbit IgG conjugated to horseradish peroxidase (dilution, 1:80,000 in TBST) (Protein Tech Group, Inc., Chicago, IL, USA), and visualized by enhanced chemiluminescence using ECL plus Western blot detection reagent (PierceTM, Rockford, IL, USA) according to the manufacturer’s instructions.
Goat anti rabbit igg conjugated to horseradish peroxidase
Manufactured by Proteintech
Sourced in United States
Goat anti-rabbit IgG conjugated to horseradish peroxidase is a secondary antibody used to detect the presence of rabbit primary antibodies in various immunoassays. It consists of goat-derived antibodies that specifically bind to rabbit immunoglobulin G (IgG) molecules, with the addition of a horseradish peroxidase enzyme label.
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Lab products found in correlation
2 protocols using goat anti rabbit igg conjugated to horseradish peroxidase
1
Western Blot Analysis of Recombinant Proteins
2
SDS-PAGE and Western Blot Protocol
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SDS-PAGE and western blots were performed as previously described (Yu et al., 2015 (link)) with minor modifications. Briefly, cell pellets were resolved by SDS-PAGE using 15% gels. Gels were either stained with Coomassie brilliant blue G-250 for confirming the bands of fusion proteins or processed for western blotting by electro-transferring the proteins onto 0.2 μm nitrocellulose membrane (Whatman GmbH, Dossel, Germany). Membranes were then blocked with 5% (w/v) skimmed milk powder in phosphate-buffered saline (PBS), incubated sequentially with RAH01 (dilution, 1:4000) and goat anti-rabbit IgG conjugated to horseradish peroxidase (dilution, 1:80,000) (Protein Tech Group, Inc., Chicago, USA), and visualized by enhanced chemiluminescence using ECL plus western blot detection reagents (GE Healthcare, Chalfont St Giles, UK) according to the manufacturer’s instructions.
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