Human gastric cancer cells were transfected with siRNA (scRNA or SPON2 siRNA) and vector plasmid (pCMV-SPORT6_E.V or pCMV-SPORT6_SPON2). After 24 h transfection, 2 × 104 cells/200 µL FBS-free medium was added to the trans-well upper chamber (Corning Costar, Cambridge, MA, USA) on a filter coated with 0.5 mg/mL collagen type I (BD Biosciences, Seoul, Korea) for migration assay. For the invasion assay, the chamber was coated with Matrigel (1:15) (BD Biosciences). RPMI 1640 medium containing 10% FBS and 1% antibiotics was added to the lower chamber, and plates were incubated for 20 h. Cells that migrated and invaded were visualized after hematoxylin and eosin staining. For quantification, cells were counted from five randomly selected areas in each well using wide-field microscopy. Data were expressed as mean ± standard error of the mean (SEM) from three independent experiments.
Matrigel 1 15
Manufactured by BD
Matrigel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is used as a cell culture substrate that provides a reconstituted extracellular matrix environment to support the growth and differentiation of various cell types.
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Lab products found in correlation
2 protocols using matrigel 1 15
1
Spondin-2 Regulates Gastric Cancer Cell Migration and Invasion
2
Investigating Cancer Cell Migration and Invasion
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AGS and MKN28 cells were transfected with siRNA (scRNA or HSF1 siRNA) and vector plasmid (pcDNA_EV or pcDNA_HSF1). After 24-hour transfection, 1×104 cells from each well were isolated and added to the upper transwell chamber (Corning Costar, Tewksbury, MA, USA) that carried a filter coated with 0.5 mg/mL of collagen type I (BD Biosciences, Seoul, Korea) for the migration assay or a filter coated with Matrigel (1:15) (BD Biosciences) for the invasion assay. RPMI-1640 containing 10% FBS and 1% antibiotics was added to the lower chamber, and the plates were incubated for 20 hours. Cells that migrated and invaded were quantified after hematoxylin and eosin staining, as previously described.18 (link) For quantification, cells were counted in five randomly selected areas of each well using wide-field microscopy. Data are expressed as mean±SEM from three independent experiments.
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