NCM460 cells were treated with CPUY192018 (10 μM) at indicated times, then incubated at 4 °C overnight with Nrf2 primary antibodies (abcam, UK). After washing with PBS, cells were incubated at 37 °C for 1 h with FITC-labeled secondary goat anti-rabbit IgG antibody (Life Technology). Cells were then stained with fluorochrome dye DAPI (Santa Cruz Biotechnology, Santa Cruz, CA) to visualize the nuclei and observed under a laser scanning confocal microscope (Olympus Fluoview FV1000, Japan) with a peak excitation wave length of 570 nm and 340 nm.
Fitc labeled secondary goat anti rabbit igg antibody
Manufactured by Thermo Fisher Scientific
The FITC-labeled secondary goat anti-rabbit IgG antibody is a laboratory reagent used for the detection and visualization of rabbit primary antibodies in various immunochemical techniques, such as immunofluorescence, Western blotting, and ELISA. The antibody is conjugated with the fluorescent dye FITC (fluorescein isothiocyanate), allowing for the specific detection of rabbit immunoglobulin G (IgG) molecules.
Automatically generated - may contain errors
2 protocols using fitc labeled secondary goat anti rabbit igg antibody
1
Nrf2 Activation Assay in NCM460 Cells
2
Nrf2 Localization in HK-2 Cells
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HK-2 cells were treated with CPUY192018 (10 μM) at indicated times, then incubated at 4 °C overnight with Nrf2 primary antibodies (abcam, UK). After washing with PBS, cells were incubated at 37 °C for 1 h with FITC-labeled secondary goat anti-rabbit IgG antibody (Life Technology). Cells were then stained with fluorochrome dye DAPI (Santa Cruz Biotechnology, Santa Cruz, CA) to visualize the nuclei and observed under a laser scanning confocal microscope (Olympus Fluoview FV1000, Japan) with a peak excitation wave length of 570 nm and 340 nm.
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