Anti rat cd45 pe

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Anti-Rat CD45 PE is a laboratory reagent used for the detection and identification of CD45-expressing cells in rat samples. It is a fluorochrome-conjugated monoclonal antibody that binds specifically to the CD45 cell surface antigen. This product is intended for research use only and its performance characteristics have not been established for diagnostic or clinical procedures.

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Lab products found in correlation

Anti rat cd8a apc, by Thermo Fisher Scientific (3 mentions) Anti rat cd44 pe, by Thermo Fisher Scientific (3 mentions) Epics xl, by Beckman Coulter (3 mentions) Ab32529, by Abcam (2 mentions) Ab87540, by Abcam (2 mentions) Anti rat cd62l, by BioLegend (2 mentions) Ab91109, by Abcam (1 mentions)

3 protocols using anti rat cd45 pe

Isolation and Characterization of CD8+ T-cells

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Mononuclear cells were isolated from blood samples and counted. Cells were then labeled with the following fluorescence-conjugated monoclonal antibodies: anti-Rat CD45 PE, anti-Rat CD8a APC, anti-Rat CD44 PE, and anti-Rat CD62L (eBioscience, San Diego, CA, USA). CD8⁺ T-cells and memory CD8⁺ T-cells were sorted by flow cytometry (EPICS-XL, Beckman-Coulter, USA).

Cui N., Su L.X., Wang H., Xiao M., Yang F., Zheng M., Li X., Xu Y.C, & Liu D.W. (2016). mTOR Modulates Lymphocyte Differentiation through T-bet and Eomesodermin in Response to Invasive Pulmonary Aspergillosis in Rats. Chinese Medical Journal, 129(14), 1704-1710.

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Isolation and Characterization of Rat CD8+ Tem Cells

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Peripheral blood mono-nuclear cells (PBMC) were isolated from blood samples and counted by flow cytometry. Cells were then labeled with the following fluorescence-conjugated monoclonal antibodies: anti-Rat CD45 PE (12-0451-81, eBioscience, San Diego, CA, USA), anti-Rat CD8a APC (17-0081-81, eBioscience), anti-Rat CD44 PE (25-0441-81, eBioscience), anti-Rat CD62L (104432, Biolegend). CD8⁺Tem cells were sorted by flow cytometry (EPICS-XL, Beckman-Coulter, USA), then stained for IFN-γ (11-7311-81, eBioscience), mTOR (ab87540, Abcam, Cambridge, MA), S6K (ab32529, Abcam), T-bet (ab91109, Abcam), and Eomes (53-4875-82, eBioscience) expression.

Wang H., Li J., Han Q., Yang F., Xiao Y., Xiao M., Xu Y., Su L., Cui N, & Liu D. (2017). IL-12 Influence mTOR to Modulate CD8+ T Cells Differentiation through T-bet and Eomesodermin in Response to Invasive Pulmonary Aspergillosis. International Journal of Medical Sciences, 14(10), 977-983.

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Isolation and Characterization of CD8+ Tem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peripheral blood mononuclear cells were isolated from blood samples and counted by flow cytometry. Cells were then labeled with the following fluorescence-conjugated monoclonal antibodies: anti-rat CD45 PE (12-0451-81, eBioscience, San Diego, CA, USA), anti-rat CD8a APC (17-0081-81, eBioscience), anti-rat CD44 PE (25-0441-81, eBioscience), and anti-rat CD62L (104432, Biolegend). CD8⁺ Tem were sorted by flow cytometry (EPICS-XL, Beckman-Coulter, Indianapolis, IN, USA) and then stained for interferon (IFN)-γ (11-7311-81, eBioscience), mTOR (ab87540, Abcam, Cambridge, MA, USA), and S6K (ab32529, Abcam) expression.

Cui N., Wang H., Su L.X., Zhang J.H., Long Y, & Liu D.W. (2017). Role of Triggering Receptor Expressed on Myeloid Cell-1 Expression in Mammalian Target of Rapamycin Modulation of CD8+ T-cell Differentiation during the Immune Response to Invasive Pulmonary Aspergillosis. Chinese Medical Journal, 130(10), 1211-1217.

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