The primary antibodies that were used in this study are: anti-Gfap monoclonal antibody (Sigma), anti-Gfap polyclonal antibody (Abcam), anti-Slc1a3 (EAAT1) polyclonal antibody (Abcam), anti-Aldh1l1 polyclonal antibody (Abcam), chicken anti-Map2 antibody (Abcam), anti-Rbfox3 (NeuN) monoclonal antibody (EMD Millipore), and anti-beta III Tubulin (Tuj1) monoclonal antibody (Abcam). For mouse antibody (monoclonal antibody) on mouse tissues, the endogenous mouse IgG blocking method was used to reduce background straining. Briefly, after normal serum blocking and before primary antibody incubation, the mouse brain sections were incubated with unconjugated affiniPure Fab fragment goat Anti-Mouse IgG (H + L) (Jackson ImmunoResearch Labs, West Grove, PA) for 1 hr at room temperature. Fluorophore conjugated secondary antibodies were purchased from Invitrogen (Molecular Probe). Images were taken with a Zeiss LSM510 confocal microscope, processed with software NIH ImageJ and Imaris (Bitplane), and composed with adobe Photoshop.
Anti gfap polyclonal antibody
Manufactured by Abcam
Sourced in United States
Anti-Gfap polyclonal antibody is a laboratory reagent used for the detection and analysis of the Glial Fibrillary Acidic Protein (GFAP) in biological samples. GFAP is an intermediate filament protein that is primarily expressed in astrocytes, a type of glial cell in the central nervous system.
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2 protocols using anti gfap polyclonal antibody
1
Multiplex Immunostaining of Neuronal Markers
2
Spinal Cord Immunohistochemistry Analysis
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The L4-L5 segment of the spinal cord was paraffin embedded and sectioned at a thickness of 4 μm. Six sections from each rat, at least 200 μm apart, were processed in a blinded manner. The staining was carried out as previously described. 9,10 Antibodies used were as follows: anti-GFAP polyclonal antibody (1:1000 diluted, Abcam, Cambridge, MA, USA), anti-Iba1 polyclonal antibody (1:1000 diluted, Abcam) and biotinylated goat secondary antibody (1:100 diluted, Santa Cruz Biotechnology, Dallas, TX, USA). Five different vision fields of each section from different groups were analyzed by an image analysis collection system (Q553Cw; Leica, Wetzlar, Germany), and average optical densities (gray scale) were measured.
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