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Cresyl violet

Manufactured by Bio-Optica
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Sourced in Italy

Cresyl violet is a laboratory stain commonly used for histological and cytological applications. It is a basic dye that selectively stains Nissl substance, which is found in the cytoplasm of neurons. This stain is used to visualize the structure and distribution of neurons in tissue samples.

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Lab products found in correlation

Killik, by Bio-Optica (1 mentions) Cm1900 uv, by Leica camera (1 mentions) Eukitt, by Bio-Optica (1 mentions) Bioclear, by Bio-Optica (1 mentions) Bx60 microscope, by Olympus (1 mentions) Hematoxylin and eosin, by Bio-Optica (1 mentions)

2 protocols using cresyl violet

1

Cryostat Sectioning for Brain Histology

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After decapitation, the brain was rapidly removed, and processed to prepare cryostat sections, essentially as previously described [76 (link)]. Briefly, the left/right half of the brain was embedded in killik (Bio-Optica, Milan, Italy), frozen in liquid nitrogen and sectioned using a cryostat (Leica CM1900UV, Wetzlar, Germany). 10–15 µm-thick coronal sections were collected on poly(L)-lysine-coated slides and stored at −20 °C until further processing. Selected sections were thawed at RT, stained with cresyl violet (Bio-Optica, Milan, Italy), differentiated in two changes of 95% ethanol, quickly dehydrated with absolute ethanol, cleared with Bio-clear (Bio-Optica, Milan, Italy) and permanently mounted with Eukitt (Bio-Optica, Milan, Italy). Whole-slide images were acquired using the Manual WSI software (Microvisioneer, Esslingen am Neckar, Germany) and a BX60 microscope (Olympus, Hamburg, Germany) equipped with a color camera (Basler, Ahrensburg, Germany).
Amato S., Averna M., Guidolin D., Pedrazzi M., Pelassa S., Capraro M., Passalacqua M., Bozzo M., Gatta E., Anderlini D., Maura G., Agnati L.F., Cervetto C, & Marcoli M. (2022). Heterodimer of A2A and Oxytocin Receptors Regulating Glutamate Release in Adult Striatal Astrocytes. International Journal of Molecular Sciences, 23(4), 2326.
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2

Histological Staining and Imaging

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Sections were stained/counterstained with 1% toluidine blue in 1% saline (TB), hematoxylin and eosin (HE), or cresyl violet (all from Bio-Optica, Milan, Italy), and digital images were produced with a direct E400 Eclipse light microscope (Nikon, Tokyo, Japan).
Micera A., Jirsova K., Esposito G., Balzamino B.O., Di Zazzo A, & Bonini S. (2020). Mast Cells Populate the Corneoscleral Limbus: New Insights for Our Understanding of Limbal Microenvironment. Investigative Ophthalmology & Visual Science, 61(3), 43.
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