Proteins were separated on either 4–20% gradient Tris-glycine gels (Life Technologies) or homemade 10% or 12% gels in the presence of DTT. The BLUEstain protein ladder 11–245 kDa (Gold Biotechnology) was used as a molecular weight marker. BN-PAGE was performed using the Novex NativePAGE BisTris gel system in 4–16% gradient gels according to the manufacturer’s instructions (Life Technologies). The NativeMark unstained protein standard (Life Technologies) was used as the molecular weight marker. All gels were stained with Coomassie Blue R-250 solution.
Bluestain protein ladder 11 245 kda
Manufactured by GoldBio
The BLUEstain protein ladder 11–245 kDa is a pre-stained protein standard used for estimating the molecular weights of proteins in SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting applications. It covers a wide range of molecular weights from 11 to 245 kDa.
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Lab products found in correlation
2 protocols using bluestain protein ladder 11 245 kda
1
Gel-Based Protein Separation and Analysis
2
SDS-PAGE and BN-PAGE Protein Analysis
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SDS-PAGE analyses were performed using 4–20% gradient Tris-glycine gels (Life Technologies) or homemade 10% gels in the presence (reducing) or absence (non-reducing) of DTT. The BLUEstain protein ladder 11–245 kDa (Gold Biotechnology) was used as a molecular mass marker. BN-PAGE was performed using the Novex NativePAGE BisTris gel system in 4–16% gradient gels according to the manufacturer's instructions (Life Technologies). In the case of JRFL-FD, a native 4–12% gradient Tris-glycine gel (Life Technologies) was used with Tris-glycine buffer (Bio-Rad). The NativeMark unstained protein standard (Life Technologies) was used as the molecular mass marker. All gels were stained with Coomassie Blue R-250 solution.
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