AA-2G was provided by Hayashibara Biochemical Laboratories (Okayama, Japan). Sodium ascorbate (AA-Na), heparin and calcein-AM solution were obtained from Wako Pure Chemical Industries (Osaka, Japan). RPMI 1640 medium was purchased from Corning (NY, USA). Fetal bovine serum (FBS, heat-inactivated) was from Hyclone (Logan, UT, USA). Triton X-100 was from Sigma-Aldrich (St. Louis, MO, USA). Penicillin and streptomycin solution and dithiothreitol (DTT) were purchased from Nacalai Tesque (Kyoto, Japan). All of the chemicals used were of the highest grade commercially available.
Calcein am solution
Manufactured by Fujifilm
Sourced in Japan
Calcein-AM solution is a fluorescent dye used in cell biology research. It is a cell-permeant dye that can be used to assess cell viability and proliferation. When the dye enters a live cell, intracellular esterases cleave the acetoxymethyl (AM) ester, resulting in the fluorescent calcein compound, which is retained within the cell. The fluorescence intensity is proportional to the number of live cells present, making it a useful tool for quantifying cell populations.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using calcein am solution
1
Ascorbate-Mediated Cell Viability Assay
2
Antioxidant activity evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Sodium erythorbate monohydrate (EA-Na·H2O) was obtained from Tokyo Chemical Industries (Tokyo, Japan). Sodium ascorbate (AA-Na), heparin, calcein-AM solution and 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) were purchased from Wako Pure Chemical Industries (Osaka, Japan). RPMI 1640 medium was purchased from Corning (NY, USA). Fetal bovine serum (FBS, heat-inactivated) was from HyClone (Logan, UT, USA). Triton X-100 was from Sigma (St. Louis, MO, USA). Dithiothreitol (DTT) was obtained from Nacalai tesque (Kyoto, Japan).
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