For immunofluorescence staining, cells were washed with PBS, fixed with 4% paraformaldehyde for 20 min and then permeabilized with 0.2% Triton X-100 for 8 min. The cells were then washed twice with PBS, blocked in 10% FBS for 45 min and incubated overnight with primary antibodies at 4°C. Next, the cells were stained with fluorescent secondary antibodies for 2 h and with Hoechst 33342 dye for 20 min. Images were acquired using a fluorescence microscope. The primary antibodies used in this work included anti-cTnT (Abcam), anti-N-cad (BD) and anti-GFP (Abcam).
Anti n cad
Manufactured by BD
Anti-N-cad is a laboratory instrument designed to detect and quantify the presence of N-cadherin, a cell surface protein involved in cell-cell adhesion. It utilizes a specialized detection method to accurately measure N-cadherin levels in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti n cad
1
Immunofluorescence Staining of Cells
2
iPSC Differentiation into NPCs and Neurons
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!