RHu PDGF-BB, IL-1β and imatinib mesylate were purchased from MedChemExpress, United States (cat. nos. HY-P7055; HY-P7097; HY-50946); PDGF-BB polyclonal antibody, collagen II monoclonal antibody and aggrecan neo polyclonal antibody were purchased from Thermo Fisher Scientific, United Kingdom (cat. nos. PA5-88272; MA5-12789; PA1-1746); MMP3 antibody, MMP9 antibody, Adamts4 antibody, Adamts5 antibody and Caspase1 antibody were purchased from Abcam, United Kingdom (cat. nos. ab52915; ab76003; ab185722; ab41037; ab179515); PDGFR antibody, P-PDGFR antibody, P-P38 antibody and P-JNK antibody were purchased from Hangzhou Huaan Biotechnology Co., Ltd., China (cat. nos. SN0646; R1510-44; ER2001-52; ET1601-28); P-PI3K antibody, PI3K antibody, P-AKT antibody, AKT antibody, P38 antibody, JNK antibody, P-ERK antibody, ERK antibody, β-actin antibody and NLRP3 antibody were purchased from Cell Signaling Technology, United States (cat. nos.17366; 4292; 9271; 4691; 9212S; 9252S; 4370; 4695; 4970; ab263899); IL-1β antibody was purchased from ABclonal Technology Co., Ltd., China (cat. no. A1112).
Pi3k antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The PI3K antibody is a laboratory reagent used to detect and study the phosphoinositide 3-kinase (PI3K) enzyme in biological samples. PI3K is a crucial signaling molecule involved in various cellular processes, including cell growth, proliferation, and metabolism. The antibody specifically recognizes and binds to the PI3K protein, allowing researchers to identify and quantify its presence in their experiments.
Automatically generated - may contain errors
Lab products found in correlation
Perk antibody, by Cell Signaling Technology (2 mentions)
P pi3k antibody, by Cell Signaling Technology (2 mentions)
Akt antibody, by Cell Signaling Technology (2 mentions)
P akt antibody, by Cell Signaling Technology (2 mentions)
Erk antibody, by Cell Signaling Technology (2 mentions)
Jnk antibody, by Cell Signaling Technology (2 mentions)
P38 antibody, by Cell Signaling Technology (2 mentions)
Mmp9 antibody, by Abcam (1 mentions)
Mmp3 antibody, by Abcam (1 mentions)
Nlrp3 antibody, by Cell Signaling Technology (1 mentions)
Collagen 2 monoclonal antibody, by Thermo Fisher Scientific (1 mentions)
Caspase 1 antibody, by Abcam (1 mentions)
β actin antibody, by Cell Signaling Technology (1 mentions)
Imatinib mesylate, by MedChemExpress (1 mentions)
Il 1β, by MedChemExpress (1 mentions)
Il 1β antibody, by ABclonal (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Fis1 antibody, by Proteintech (1 mentions)
Cytochrome c antibody, by Proteintech (1 mentions)
7 protocols using pi3k antibody
1
Chondroprotective Effects of PDGF, IL-1β, and Imatinib
2
Protein Expression Analysis in HK-2 Cells
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HK-2 cells (at least 1 × 10 7 cells) were collected and added to RIPA lysis buffer (Beyotime, Shanghai, China). Subsequently, cells were ultrasonically disrupted for 3 min and centrifuged at 4 °C and 12,000 g for 10 min, and the supernatant was collected. The concentration of total protein extracted was determined using a bicinchoninic acid (BCA) protein assay kit (Beyotime, Shanghai, China). Next, 20 µg of total protein was mixed with 5× loading buffer and boiled for 10 min. After 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the protein was transferred to polyvinylidene fluoride membrane. The membrane was blocked in 5% skimmed milk powder for 2 h, and then incubated with primary antibodies overnight at 4℃ on a shaker. Primary antibodies used were: CUX1 antibody (1:1000; ab309139, Abcam, UK) phosphorylated phosphoinositide 3-kinase (p-PI3K, Tyr458) antibody, (1:1000; #17,366, Cell Signaling Technology, Danvers, MA, USA); PI3K antibody, (1:1000; #4249);
3
Icariin and Ferric Ammonium Citrate Protocol
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Icariin (United States, 489-32-7) and ferric ammonium citrate (FAC, United States, 1185-57-5) were purchased from Sigma-Aldrich (Supplementary Figure S1 ). Cleaved caspase-3 (Asp175) antibody (United States, #9661), MFN2 antibody (United States, #11925), DRP1 antibody (United States, #8570), COX IV antibody (United States, #4850), RUNX-2 antibody (United States, #12556), active β-catenin antibody (United States, #19807), cyclin D1 antibody (United States, #2922), PI3K antibody (United States, #4257), P-PI3K antibody (United States, #4228), AkT antibody (United States, #4691), P-AkT antibody (United States, #4060), mTOR antibody (United States, #2972), P-mTOR antibody (United States, #5536), P-ERK antibody (United States, #4370), ERK antibody (United States, #4695), P-p38 antibody (United States, #4511), p38 antibody (United States, #8690), P-JNK antibody (United States, #4668), JNK antibody (United States, #9252) were all purchased from Cell Signaling Technology. BAX antibody (United States, 50599-2-Ig), FIS1 antibody (United States, 10956-1-AP), cytochrome C antibody (United States, 66264-1-Ig) and osteopontin (OPN) antibody (United States, 22952-1-AP) were all purchased from Proteintech (United States, 50599-2-Ig). Bcl-2 antibody was purchased from R&D system (United States, MAB8272). Anti-beta actin antibody was purchased from BOSTER (China, BM3873).
4
Plasmid Transfection and Signaling Pathway Analysis
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The transfected plasmids were purchased from GeneChem (Shanghai, China). The FGD1 antibody was purchased from Novus Biologicals Littleton (working dilution 1:1000 for Western blot and 1:200 for IHC staining), the PI3K antibody from Cell Signaling Technology (working dilution 1:1000 for Western blot), the Phospho-PI3K antibody from Abcam (working dilution 1:500 for Western blot), the AKT antibody from Proteintech (working dilution 1:1000 for Western blot), the Phospho-AKT antibody from Proteintech (working dilution 1:2000 for Western blot) and the β-actin antibody from Proteintech (working dilution 1:10,000 for Western blot).
5
Western Blot Protein Analysis
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Protein from cells and tissues was separated by SDS-PAGE and transferred to PVDF membranes as described [41 (link)]. The following primary antibodies were used for western blots: the monoclonal rabbit HER4 antibody (1:500, Santa Cruz); the HER2 antibodies and the p-HER4 antibody (1:500, Bioss); the E-cadherin, Vimentin, Cleavage-caspase3, Cleavage-caspase9, YAP1, and GAPDH antibodies (1:500, Proteintech Group); and PI3K antibodies (1:1000, Cell Signaling Technology). The proteins were visualized using the Luminata Chemiluminescent Detection Kit (Millipore).
6
Curcumin Modulates AKT/NF-κB Pathway
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Curcumin was from the National Food and Drug Testing Institute, Beijing, China. Dulbecco's modified Eagle's medium (DMEM) 1,640 (the concentration of glucose is 5.6 mmol/L) was purchased from Hyclone (South Logan, UT, USA), and fetal bovine serum (FBS) was acquired from GIBCO BRL (Grand Island, NY, USA). Endothelial cells growth factor was bought from Roche Inc. (Basel, Switzerland). Trizol and the one‐step reverse transcription polymerase chain reaction (PCR) kit were from Invitrogen (Carlsbad, CA). PI3K inhibitor LY294002, anti‐oxidant N‐acetylcysteine (NAC) and NF‐κB inhibitor pyrrolidine dithiocarbamate (PDTC) were from Sigma‐Aldrich (St. Louis, MO, USA). Rabbit NF‐κB antibody and rabbit CD 31 antibody were provided by Abcam (Cambridge, MA, USA). Rabbit monoclonal β‐actin antibody was ordered from Zhuangzhi Biotech (Xi'an, China). AKT and phospho‐AKT antibodies, 2′,7′‐dichlorodihydrofluororescein (DCF) diacetate were obtained from Beyotime (Jiangsu, China). PI3K antibodies were from Cell Signaling Technology (Danvers, MA, USA). Relative second antibodies were provided by CW Biotech (Beijing, China). Enzyme‐linked immunosorbent assay (ELISA) kits for detecting interleukin (IL)‐1β, IL‐6 and tumor necrosis factor (TNF‐α) were from West Tang (Shanghai, China). All other materials, except where indicated, were of analytical grade.
7
Molecular Mechanisms of PI3K/AKT/mTOR Signaling Pathway
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LC3B, Beclin 1, p62, phosphorylated (p)-mTOR, mTOR, p-AKT, AKT, PI3K antibodies and Ly294002, an inhibitor of the PI3K/AKT/mTOR signal pathway, were obtained from Cell Signaling Technology, Inc. GAPDH antibody was obtained from Bioworld Technology, Inc. BSA was obtained from Sigma-Aldrich; Merck KGaA. Hypochlorous acid (HOCl) was purchased from Fluka Chemie AG (Sigma-Aldrich; Merck KGaA). Tivantinib, a competitive inhibitor of HGF, and insulin-like growth factor 1 (IGF-1), an inducer of the PI3K/AKT/mTOR signal pathway, were acquired from APeXBIO Technology LLC. Recombinant human HGF (rhHGF, an analogs of HGF) was obtained from PeproTech, Inc., and the HGF ELISA kit was purchased from MultiSciences (Lianke) Biotech Co., Ltd. The Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies, Inc.
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