Coulter counter z2 particle counter

Manufactured by Beckman Coulter

The Coulter Counter Z2 is a particle counter designed to accurately measure the size and concentration of particles in a sample. It utilizes the Coulter principle, which measures changes in electrical impedance as particles pass through a small aperture, to detect and characterize particles in the size range of 0.4 to 1200 microns.

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Lab products found in correlation

Fetal calf serum (fcs), by Merck Group (2 mentions) Gentamycin, by Thermo Fisher Scientific (2 mentions) M199 medium, by Thermo Fisher Scientific (1 mentions) Hygromycin, by Thermo Fisher Scientific (1 mentions) Neomycin, by Merck Group (1 mentions) Doxycycline free fetal calf serum, by Takara Bio (1 mentions) Fetal calf serum (fcs), by Gemini Bio (1 mentions) Fetal bovine serum (fbs), by Gemini Bio (1 mentions)

Close spelling variants of this product

Z2 Coulter particle counter Z2 COULTER COUNTER Cell and Particle Counter Z2 Coulter Counter Z2 particle counter Coulter Particle Counter Coulter Z2 Z2 counter Coulter Counter Particle counter

5 protocols using coulter counter z2 particle counter

Culturing Monomorphic T. brucei BSFs and PCFs

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Monomorphic T. brucei BSFs (strain Lister 427, antigenic type MITat 1.2 clone 221a) were cultured in HMI-9 medium with 10% heat-inactivated fetal calf serum (FCS) (Sigma) at 37°C and 5% CO₂ (32 (link)). Cells of single marker (SM) (33 (link)) or 2T1 (34 (link)) background co-expressing the T7 RNA polymerase and tetracycline (Tet) repressor were used to generate the BSF cell lines for this study.
PCFs (strain 427) were cultured in modified SDM-79 with 10% heat-inactivated FCS (Sigma) at 27°C (35 (link)). Here, 29–13 or wild-type (WT) procyclic cells were used to generate transgenic procyclic cell lines. The 29–13 procyclic cells co-express T7 RNA polymerase and the Tet repressor (33 (link)).
Cell densities of BSF and PCF cultures were determined using a Coulter Counter Z2 particle counter (Beckman Coulter). Transfections and drug selections were carried out as described previously (36 (link)).

Reis H., Schwebs M., Dietz S., Janzen C.J, & Butter F. (2018). TelAP1 links telomere complexes with developmental expression site silencing in African trypanosomes. Nucleic Acids Research, 46(6), 2820-2833.

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Cultivation and Transfection of Trypanosoma brucei

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Monomorphic Trypanosoma brucei Lister 427 bloodstream form (BSF) MiTat 1.2 (clone 221a) strain, and a derivative ‘2T1’ strain [87 (link)] that contains a puromycin-tagged ribosomal spacer for directed integration of the RNAi construct and expresses a Tet repressor protein, were cultivated in HMI-9 medium [88 (link)] with 10% heat-inactivated fetal calf serum (FCS; 56°C for 1 h) at 37°C and 5% CO2. Strains were cultured with their attendant drug selection with the following concentrations used: 2.5 μg/ml G418 (neomycin), 5 μg/ml hygromycin, 0.1 μg/ml puromycin, 5 μg/ml blasticidin, 2.5 μg/ml phleomycin. RNAi was induced with 1 μg/ml tetracycline. Reduction of mRNA was monitored by RNA/FISH (S1M Fig). Growth rates were monitored for 96 h and cell densities were determined every 24 h using a Coulter Counter Z2 particle counter (Beckman Coulter). Procyclic forms (PCF; strain 427) were cultured in modified SDM-79 with 10% heat-inactivated FCS (Sigma) at 27°C. BSF and PCF parasites were transfected as previously described [89 (link)], with independent clones obtained by limiting dilution.

Vellmer T., Hartleb L., Fradera Sola A., Kramer S., Meyer-Natus E., Butter F, & Janzen C.J. (2022). A novel SNF2 ATPase complex in Trypanosoma brucei with a role in H2A.Z-mediated chromatin remodelling. PLoS Pathogens, 18(6), e1010514.

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Leishmania Species Culture and Characterization

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Promastigote-form L. mexicana (WHO strain MNYC/BZ/62/M379), L. major Friedlin, L. donovani (strain BPK190, Decuypere et al., 2005 (link)), and L. infantum (strain MHOM/FR/91/LEM2259, Sulahian et al., 1997 (link)) were grown at 28°C in M199 medium (Life Technologies) supplemented with 2.2 g/l NaHCO₃, 0.0025% haemin, 0.1 mM adenine hemisulfate, 1.2 μg/ml 6-biopterin, 40 mM 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid pH 7.4, and 20% FCS (fetal calf serum). Media were supplemented with the relevant selection drugs: 40 or 400 μg/ml Hygromycin B, 40 μg/ml Puromycin Dihydrochloride, and 40 μg/ml G-418 Disulfate. The identity of each Leishmania species was confirmed by sequencing the LPG2 locus (Akhoundi et al., 2017 (link); Figure 2—figure supplement 1) and cell lines were tested negative for mycoplasma contamination at Eurofins Genomics. Doubling times were determined by sub-culturing parasites repeatedly (over 5 days) to 10⁶ cells/ml and measuring cell densities 24 hr later using a Coulter Counter Z2 particle counter (Beckman Coulter).

Engstler M, & Beneke T. (2023). Gene editing and scalable functional genomic screening in Leishmania species using the CRISPR/Cas9 cytosine base editor toolbox LeishBASEedit. eLife, 12, e85605.

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Culturing Procyclic Trypanosoma brucei

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Cells used in all experiments were either procyclic T. brucei brucei strain 427 or the doxycycline-inducible strain 29-13. 427-based cell lines were cultured at 27 °C in Beck’s Medium (Hyclone, GE Healthcare, Logan, Utah) supplemented with 500 μg/mL penicillin-streptomycin-glutamine (Hyclone), 10% fetal calf serum (Gemini Bioproducts, West Sacramento, CA), and 10 μg/mL gentamycin (ThermoFisher Scientific, Waltham, MA). 29-13-based lines were cultured at 27°C in Beck’s Medium supplemented with 500 μg/mL penicillin-streptomycin-glutamine, 15% doxycycline-free fetal calf serum (Clontech), 10 μg/mL gentamycin, 50 μg/mL hygromycin (ThermoFisher Scientific), and 15 μg/mL neomycin (Sigma-Aldrich, St. Louis, MO). Cell concentration was determined using a Z2 Coulter Counter particle counter (Beckman Coulter, Brea, CA).

Hilton N.A., Sladewski T.E., Perry J.A., Pataki Z., Sinclair-Davis A.N., Muniz R.S., Tran H.L., Wurster J.I., Seo J, & de Graffenried C.L. (2018). Identification of TOEFAZ1-interacting proteins reveals key regulators of Trypanosoma brucei cytokinesis. Molecular microbiology, 109(3), 306-326.

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Culturing Monomorphic Procyclic T. brucei

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Cells used in all experiments were monomorphic procyclic form T. brucei Lister strain 427 or a tetracycline-inducible strain 427-based strain generated by introducing the Single Marker Oxford plasmid [30 (link)]. 427-based cell lines were grown at 27°C in Beck’s Medium (Hyclone, Logan, Utah) supplemented with 500 μg/mL penicillin-streptomycin-glutamine (Hyclone), 10% fetal bovine serum (Gemini Bioproducts, West Sacramento, CA), and 10 μg/mL gentamycin (ThermoFisher Scientific, Waltham, MA). SmOx-based cell lines were cultured at 27°C in SmOx medium comprised of Beck’s Medium supplemented with 500 μg/mL penicillin-streptomycin-glutamine, 1 μg/mL puromycin, 10% tetracycline-free fetal bovine serum (RnD Systems, Minneapolis, MN), and 10 μg/mL gentamycin. Cell counts were determined using a Z2 Coulter Counter particle counter (Beckman Coulter, Brea, CA).

Muniz R.S., Campbell P.C., Sladewski T.E., Renner L.D, & de Graffenried C.L. (2022). Revealing spatio-temporal dynamics with long-term trypanosomatid live-cell imaging. PLoS Pathogens, 18(1), e1010218.

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