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Axopatch 1b

Manufactured by Molecular Devices
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The Axopatch 1B is a laboratory instrument designed for electrophysiological recordings. It is used to measure and amplify electrical signals from a wide range of biological preparations, including single cells, tissues, and ion channels. The Axopatch 1B provides a stable and accurate voltage-clamp or current-clamp amplifier for these types of electrophysiological experiments.

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Lab products found in correlation

Pc 10 vertical puller, by Narishige (2 mentions)

Close spelling variants of this product

Axopatch 1B amplifier (4 citations)

2 protocols using axopatch 1b

1

Single-Channel Analysis of ENaC Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
ENaC activity was recorded using standard single channel patch clamp analysis in the cell-attatched configuration. Micropipettes were pulled with a two-stage Narishige PC-10 vertical puller (Narishige International, Amityville, NY) from filamented borosilicate glass capillaries purchased from World Precision Instruments (Sarasota, FL). Micropipette resistances were between 5–9 MΩ when filled and immersed in patch solution composed of (in mM): 140 NaCl, 5 KCl,1 CaCl2, 1 MgCl2, and 10 HEPES, adjusted to pH 7.40 with NaOH. Channel currents were sampled at 1 kHz with an Axopatch 1B patch amplifier (Molecular Devices) and filtered at 200 Hz with a low-pass Bessel filter. Continuous single channel activity was recorded and then analyzed using Pclamp 10 software for channel open probability (Po) and number (N) of active channels in SAEC apical membrane. Chord conductances (γ), were calculated from hyperpolarized and depolarized potentials.
Grant G.J., Coca C., Zhao X.M, & Helms M.N. (2020). Oxidized Glutathione Increases Delta-Subunit Expressing Epithelial Sodium Channel Activity in Xenopus laevis Oocytes. eMedical research, 2, 100008.
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2

Single-Channel Analysis of ENaC Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
ENaC activity was recorded using standard single channel patch clamp analysis in the cell-attatched configuration. Micropipettes were pulled with a two-stage Narishige PC-10 vertical puller (Narishige International, Amityville, NY) from filamented borosilicate glass capillaries purchased from World Precision Instruments (Sarasota, FL). Micropipette resistances were between 5–9 MΩ when filled and immersed in patch solution composed of (in mM): 140 NaCl, 5 KCl,1 CaCl2, 1 MgCl2, and 10 HEPES, adjusted to pH 7.40 with NaOH. Channel currents were sampled at 1 kHz with an Axopatch 1B patch amplifier (Molecular Devices) and filtered at 200 Hz with a low-pass Bessel filter. Continuous single channel activity was recorded and then analyzed using Pclamp 10 software for channel open probability (Po) and number (N) of active channels in SAEC apical membrane. Chord conductances (γ), were calculated from hyperpolarized and depolarized potentials.
Grant G.J., Coca C., Zhao X.M, & Helms M.N. (2020). Oxidized Glutathione Increases Delta-Subunit Expressing Epithelial Sodium Channel Activity in Xenopus laevis Oocytes. eMedical research, 2, 100008.
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