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Peptide pool and brefeldin a

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Peptide pool and brefeldin A are laboratory reagents used in cell biology research. Peptide pool is a mixture of small peptide fragments, while brefeldin A is a fungal metabolite. Both are commonly used as tools to study cellular processes, but their specific functions and applications can vary depending on the research context.

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Lab products found in correlation

Facsverse, by BD (2 mentions) Cytofix cytoperm solution, by BD (2 mentions) Anti mouse cd16 32 percp cy5.5 clone 93, by BioLegend (2 mentions) Anti mouse tnf pe clonemp6 xt22, by Thermo Fisher Scientific (2 mentions) Anti mouse cd4 efluor 450 clonerm4 5, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Brefeldin A (612 citations)

2 protocols using peptide pool and brefeldin a

1

Multiparameter Flow Cytometry of T-cell Responses

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The following monoclonal antibodies were used: rat anti-mouse CD8α-Alexa 488 (clone 53-6.7, Cat. No.: 100723, Biolegend, San Diego, CA), anti-mouse CD16/32-PerCP-Cy5.5 (clone 93, Cat. No.: 45-0161-82, eBioscience, San Diego, CA), anti-mouse CD4-eFluor 450 (cloneRM4-5, Cat. No.: 48-0042-82 eBioscience, San Diego, CA), anti-mouse TNF-PE (cloneMP6-XT22, Cat. No.: 12-7321-82Bioscience, San Diego, CA), anti-mouse IFN-γ-APC (cloneXMG1.2, Cat. No.: 17-7311-82, eBioscience, San Diego, CA), and mouse anti-C9 antibody (clone Rho 1D4, Cat. No.: MAB5356, EMD Millipore). For intracellular cytokine staining (ICS), lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers, fixed/permeabilized with Cytofix/Cytoperm Solution (BD Biosciences), and labeled with anti-IFN-γ and anti-TNF antibody. All flow cytometry data were acquired on a BD FACSVerse and were analyzed using FlowJo software.
Sun J., Zhuang Z., Zheng J., Li K., Wong R.L., Liu D., Huang J., He J., Zhu A., Zhao J., Li X., Xi Y., Chen R., Alshukairi A.N., Chen Z., Zhang Z., Chen C., Huang X., Li F., Lai X., Chen D., Wen L., Zhuo J., Zhang Y., Wang Y., Huang S., Dai J., Shi Y., Zheng K., Leidinger M.R., Chen J., Li Y., Zhong N., Meyerholz D.K., McCray PB J.r., Perlman S, & Zhao J. (2020). Generation of a Broadly Useful Model for COVID-19 Pathogenesis, Vaccination, and Treatment. Cell, 182(3), 734-743.e5.
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2

Multicolor Flow Cytometry for Cytokine Profiling

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The following monoclonal antibodies were used: rat anti-mouse CD8α-Alexa 488 (clone 53-6.7, Cat. No. 100723, Biolegend, San Diego, CA), anti-mouse CD16/32-PerCP-Cy5.5 (clone 93, Cat. No. 45-0161-82, eBioscience, San Diego, CA), anti-mouse CD4-eFluor 450 (cloneRM4-5, Cat. No. 48-0042-82 eBioscience, San Diego, CA), anti-mouse TNF-PE (cloneMP6-XT22, Cat. No. 12-7321-82Bioscience, San Diego, CA), anti-mouse IFN-γ-APC (cloneXMG1.2, Cat. No. 17-7311-82, eBioscience, San Diego, CA), mouse anti-Flag antibody (Cat: A01429, Genscript, Nanjing), mouse anti-C9 antibody (clone Rho 1D4, Cat. No. MAB5356, EMD Millipore), anti-mouse CD3-APC (clone 145-2C11, Cat. No. 100312, eBioscience, San Diego, CA), anti-mouse CD45-PE (clone 30-F11, Cat. No. 103105, eBioscience, San Diego, CA), live-dead-BV510 (Cat. No. L23105, Thermo Scientific, Massachusetts, CA).
For intracellular cytokine staining (ICS), lymphocytes were cultured in 96-well dishes at 37 °C for 5 to 6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers, fixed/permeabilized with Cytofix/Cytoperm Solution (BD Biosciences), and labeled with anti-IFN-γ and anti-TNF antibodies. All flow cytometry data were acquired on a BD FACSVerse and were analyzed using FlowJo software.
Liu D., Chen C., Chen D., Zhu A., Li F., Zhuang Z., Mok C.K., Dai J., Li X., Jin Y., Chen Z., Sun J., Wang Y., Li Y., Zhang Y., Wen L., Zhang Z., Zhuo J., Wang J., Ran W., Wang D., Zhang S., Tang Y., Li S., Lai X., Wei P., Yuan J., Chen F., Huang S., Sun F., Qian Z., Tan W., Zhao J., Peiris M, & Zhao J. (2023). Mouse models susceptible to HCoV-229E and HCoV-NL63 and cross protection from challenge with SARS-CoV-2. Proceedings of the National Academy of Sciences of the United States of America, 120(4), e2202820120.
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