Human leukocytes were collected as described previously [36 (link)], then incubated with different concentration of Leukocins (HlgAB, HlgBC, LukSF and LukED) at room temperature (RT) for 1 h. After incubation, cells were washed twice with PBS, then stained for 30 min at RT with PE-conjugated CD11b (Biolegend, 101,207) and PerCP-conjugated CD14 (Biolegend, 325,632). The stained cells were analyzed by flow cytometry (BD FACSCalibur, San Jose, USA), and the percentage of survival cells from each sample was calculated.
Pe conjugated cd11b
Manufactured by BioLegend
Sourced in United States
PE-conjugated CD11b is a fluorescently-labeled antibody that binds to the CD11b antigen, also known as the integrin alpha M chain or Mac-1 alpha chain. CD11b is expressed on the surface of various immune cells, including monocytes, macrophages, and granulocytes. The PE (phycoerythrin) fluorescent dye is conjugated to the CD11b antibody, allowing for the detection and analysis of CD11b-expressing cells using flow cytometry or other fluorescence-based techniques.
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Lab products found in correlation
Facscalibur, by BD (1 mentions)
Fitc conjugated f4 80, by BioLegend (1 mentions)
Pe cy7 conjugated ly6g, by BioLegend (1 mentions)
Pacific blue conjugated ly6c, by BioLegend (1 mentions)
Celltrace cfse cell proliferation kit, by Thermo Fisher Scientific (1 mentions)
Percp cy5.5 conjugated cd11b, by BioLegend (1 mentions)
Fitc conjugated ifn γ, by BioLegend (1 mentions)
2 protocols using pe conjugated cd11b
1
Leukocytes Survival Assay with Leukocins
2
Immunophenotyping of Immune Cells
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Flow cytometric analysis of cells cultured in vitro and cells obtained from tissues was carried out as described previously.16 (link) Briefly, intracellular cytokine staining was measured using the Mouse IL-10 Secretion Assay-Detection Kits purchased from Miltenyi Biotec (Bergisch Gladbach, Germany), cell surface molecules were examined using anti-mouse antibodies including APC-conjugated CD80, PE-conjugated CD86 (Miltenyi Biotec), and PE-conjugated, CD45, PerCP/Cy5.5-conjugated CD11b, PE-conjugated CD11b, PE/Cy7-conjugated Ly6G, Pacific Blue-conjugated Ly6C, FITC-conjugated F4/80, Brilliant Violet 510-conjugated MHC II, FITC-conjugated IFN-γ (BioLegend, San Diego, CA, USA), and T cell proliferation was evaluated using a CellTrace™ CFSE Cell Proliferation Kit (Invitrogen, Thermo Fisher Scientific).
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