Diamat automated glycosylated hemoglobin analyzer

All procedures were performed following a 9–12 hour overnight fast and all subjects were told to consume a bland diet before blood testing. Blood pressure (BP) was measured using an OMRON HEM-7000 electronic sphygmomanometer (OMRON HealthCare, Kyoto, Japan) after the participant had rested for ≥10 min. Blood was drawn from the antecubital vein of the arm. Total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were measured with a Beckman AU Series Automatic Biochemical Analyzer (Japan), using Sekisui Medical (Japan) reagents. Fasting blood glucose (FBG), uric acid (UA), creatine (Cr), and blood urea nitrogen (BUN) were measured with the same instrument, using Beckman AU reagents. HbA1c was examined by high-performance liquid chromatography (HPLC) on a Bio-Rad Diamat automated glycosylated hemoglobin analyzer (USA).

Fasting venous blood was drawn from all subjects into EDTA vacutainers. After centrifugation, the serum and leukocyte cells were separated into new tubes for biochemical measurements and DNA analysis, respectively, and stored at –80°C until used. Biochemical variables, including serum total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were measured with a Beckman AU Series Automatic Biochemical Analyzer (Japan), using Sekisui Medical (Japan) reagents. FBG, apolipoprotein A1 (ApoA1), apolipoprotein B (ApoB), and high-sensitivity C-reactive protein (hs-CRP) were measured with the same instrument, using Beckman AU reagents, and homocysteine (Hcy) was examined using Beijing Leadman reagents. HbA1c was examined by high-performance liquid chromatography (HPLC) on a Bio-Rad Diamat automated glycosylated hemoglobin analyzer (USA). Coefficients of variation were < 10% for Hcy, hs-CRP, and < 5% for all other variables.