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Green fluorescent protein expression plasmid

Manufactured by Takara Bio
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The Green Fluorescent Protein (GFP) expression plasmid is a DNA construct designed to produce the GFP protein in host cells. GFP is a naturally occurring fluorescent protein derived from the jellyfish Aequorea victoria. The plasmid contains the GFP gene under the control of a promoter, allowing for the expression and production of the GFP protein in the host cell system.

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Lab products found in correlation

Actin mab1501r, by Merck Group (2 mentions) Geneticin 1 10 phenanthroline monohydrate, by Merck Group (2 mentions) Anti alkaline phosphatase monoclonal antibody clone 8b6, by Merck Group (2 mentions) Pacs 2 sirna, by Qiagen (2 mentions) Pacs 2 antibody 19508 1 ap, by Proteintech (2 mentions) Pegfr ab40815, by Abcam (2 mentions) Egfr 06 847, by Merck Group (2 mentions) Adam9 af949, by R&D Systems (2 mentions) Adam17 mab9301, by R&D Systems (2 mentions) Halt phosphatase inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) Complete edta free inhibitor cocktail, by Roche (2 mentions) Interferin transfection reagent, by Polyplus Transfection (2 mentions)

2 protocols using green fluorescent protein expression plasmid

1

PACS-2 Regulation of ADAM17-Mediated EGFR Signaling

Cited 1 time
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PMA and BB-94 were from Calbiochem. Geneticin, 1,10-phenanthroline monohydrate, MISSION Universal Negative Control siRNA #1, and anti-alkaline phosphatase monoclonal antibody (clone 8B6) were from Sigma-Aldrich. HALT Phosphatase Inhibitor Cocktail was from Thermo Scientific. Complete EDTA-free inhibitor cocktail was from Roche. Interferin transfection reagent was from PolyPlus, and siGENOME non-targeting siRNA pool #2, SMARTpool PACS-2 and ADAM17 siRNAs were from Dharmacon (Thermo Scientific). We additionally used PACS-2 siRNA from Qiagen and pooled three siRNAs (PACS2_6, PACS2_7 and PACS2_9). Recombinant murine TNF-α was from Strathmann Biotec AG. PACS-2 antibody (19508-1-AP) was from Proteintech. ADAM17 (ab39162), ADAM17 (ab2051), MT1-MMP (ab38971), ADAM10 (ab1997), and pEGFR (ab40815) antibodies were from Abcam. PACS-1 antibody 17703 and PACS-2 antibody 18193 were previously described53 (link),54 (link). Actin (MAB1501R) and EGFR (06–847) antibodies were from Millipore. ADAM9 (AF949), and ADAM17 (MAB9301) antibodies were from R&D Systems. PACS-2-HA expression plasmid was in a pcDNA.3 backbone40 (link) (Invitrogen), and green fluorescent protein (GFP) expression plasmid was from Clontech.
Dombernowsky S.L., Samsøe-Petersen J., Petersen C.H., Instrell R., Hedegaard A.M., Thomas L., Atkins K.M., Auclair S., Albrechtsen R., Mygind K.J., Fröhlich C., Howell M., Parker P., Thomas G, & Kveiborg M. (2015). The sorting protein PACS-2 promotes ErbB signalling by regulating recycling of the metalloproteinase ADAM17. Nature communications, 6, 7518.
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2

PACS-2 Regulation of ADAM17-Mediated EGFR Signaling

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
PMA and BB-94 were from Calbiochem. Geneticin, 1,10-phenanthroline monohydrate, MISSION Universal Negative Control siRNA #1, and anti-alkaline phosphatase monoclonal antibody (clone 8B6) were from Sigma-Aldrich. HALT Phosphatase Inhibitor Cocktail was from Thermo Scientific. Complete EDTA-free inhibitor cocktail was from Roche. Interferin transfection reagent was from PolyPlus, and siGENOME non-targeting siRNA pool #2, SMARTpool PACS-2 and ADAM17 siRNAs were from Dharmacon (Thermo Scientific). We additionally used PACS-2 siRNA from Qiagen and pooled three siRNAs (PACS2_6, PACS2_7 and PACS2_9). Recombinant murine TNF-α was from Strathmann Biotec AG. PACS-2 antibody (19508-1-AP) was from Proteintech. ADAM17 (ab39162), ADAM17 (ab2051), MT1-MMP (ab38971), ADAM10 (ab1997), and pEGFR (ab40815) antibodies were from Abcam. PACS-1 antibody 17703 and PACS-2 antibody 18193 were previously described53 (link),54 (link). Actin (MAB1501R) and EGFR (06–847) antibodies were from Millipore. ADAM9 (AF949), and ADAM17 (MAB9301) antibodies were from R&D Systems. PACS-2-HA expression plasmid was in a pcDNA.3 backbone40 (link) (Invitrogen), and green fluorescent protein (GFP) expression plasmid was from Clontech.
Dombernowsky S.L., Samsøe-Petersen J., Petersen C.H., Instrell R., Hedegaard A.M., Thomas L., Atkins K.M., Auclair S., Albrechtsen R., Mygind K.J., Fröhlich C., Howell M., Parker P., Thomas G, & Kveiborg M. (2015). The sorting protein PACS-2 promotes ErbB signalling by regulating recycling of the metalloproteinase ADAM17. Nature communications, 6, 7518.
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