The larvae were prepared as described in the previous section. Primary antibodies were rabbit anti-Harmonin (Ush1c,
Novus Biologicals), rabbit anti-Ush1g (Novus Biologicals, 1:500), mouse anti-Ush1g (Santa Cruz Biotechnology), goat anti-Ush1g
(Santa Cruz Biotechnology), Rabbit anti-Cdh23 (Sigma), goat anti-Cdh23 (Santa Cruz Biotechnology), mouse anti-Myo7a (Santa
Cruz Biotechnology). All primary antibodies were used at 1:500. After washing off the primary antibodies in PBST, the larvae
were incubated 1 hour at 37°C in PBST, incubated for 4 hours at 37°C with the Duo-Link ligation solution (Olink
Bioscience) and then overnight in Duo-Link amplification solution (Olink Bioscience).
Images of immunolabeled larvae were acquired using a Zeiss LSM 5 confocal microscope.
Novus Biologicals), rabbit anti-Ush1g (Novus Biologicals, 1:500), mouse anti-Ush1g (Santa Cruz Biotechnology), goat anti-Ush1g
(Santa Cruz Biotechnology), Rabbit anti-Cdh23 (Sigma), goat anti-Cdh23 (Santa Cruz Biotechnology), mouse anti-Myo7a (Santa
Cruz Biotechnology). All primary antibodies were used at 1:500. After washing off the primary antibodies in PBST, the larvae
were incubated 1 hour at 37°C in PBST, incubated for 4 hours at 37°C with the Duo-Link ligation solution (Olink
Bioscience) and then overnight in Duo-Link amplification solution (Olink Bioscience).
Images of immunolabeled larvae were acquired using a Zeiss LSM 5 confocal microscope.