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Las application suite 3

Manufactured by Leica

The LAS application suite 3.8 software is a comprehensive imaging and analysis platform developed by Leica. It provides a suite of tools for capturing, processing, and analyzing images from a variety of Leica microscopy and imaging systems. The software supports a range of functionalities, including image acquisition, tiling, stitching, and the application of various filters and analysis algorithms.

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2 protocols using las application suite 3

1

Dextran Perfusion Visualization

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72 hours post-fertilisation, 5 nl of Texas-Red® tagged 70 kDa dextran (2 ng/ml in PBS) was injected into the heart of tg(fli1:EGFP) embryos. Sequential images of the living fish were taken 1, 24 and 48 hours post injection (hpi) using an inverted microscope (Leica DMI 6000B) with a camera (Leica DFC420 C) and the Leica LAS application suite 3.8 software. Maximum fluorescence intensity in the heart area was measured using NIH’s ImageJ application (Supplementary Figure 3). The fluorescence values were compared in relative units of brightness for each fish.
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2

Tracing Dextran Dynamics in Zebrafish

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At 72 h post-fertilization, 5 nl of Texas-Red® tagged 70 kDa dextran (2 ng/ml in PBS) was injected into the heart of zebrafish embryos. Sequential images of the living fish were taken 1, 24, and 48 h post injection (hpi) using an inverted microscope (Leica DMI 6000 B) with a camera (Leica DFC420 C) and the Leica LAS application suite 3.8 software [35] (link).
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