The differentiated cells in T12.5 flask were dissociated into single cells using 0.05% trypsin supplemented with 0.1% EDTA. The cells were washed, resuspended in a FACS washing buffer (PBS with 5% BSA and 2.5 mM EDTA) and then stained with the desired antibodies. The antibodies used in our study were: PE Mouse Anti-Human CD31 (1:25, BD), FITC Mouse Anti-Human CD34 (1:25, BD), APC Mouse Anti-Human CD34 (1:25, BD), APC Mouse Anti-Human CD43 (1:25, BD), APC Mouse Anti-Human CD45 (1:25, BD), FITC-conjugated mouse IgG2a (1:25, BD), APC-conjugated mouse IgG1 (1:25, BD) and PE-conjugated mouse IgG1κ (1:25, BD) were used as isotype-matched negative controls. Flow cytometry was performed on Calibur flow cytometer (BD) or CytoFLEX V2-B4-R2 Flow Cytometer (BECKMAN COULTER), and the data was analyzed using FlowJo software, version 10.0.7.
Apc mouse anti human cd43
Manufactured by BD
The APC Mouse Anti-Human CD43 is a laboratory equipment product that detects the CD43 protein expressed on the surface of human cells. CD43 is a sialoglycoprotein involved in various cellular functions. The product provides a means to identify and quantify CD43-positive cells through flow cytometry or other immunoassay techniques.
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Lab products found in correlation
Apc mouse anti human cd34, by BD (2 mentions)
Pe mouse anti human cd31, by BD (2 mentions)
Calibur flow cytometer, by BD (1 mentions)
Fitc conjugated mouse igg2a, by BD (1 mentions)
Pe conjugated mouse igg1κ, by BD (1 mentions)
Fitc mouse anti human cd34, by BD (1 mentions)
Apc mouse anti human cd45, by BD (1 mentions)
Cytoflex v2 b4 r2 flow cytometer, by Beckman Coulter (1 mentions)
Apc conjugated mouse igg1, by BD (1 mentions)
Accutase, by Thermo Fisher Scientific (1 mentions)
Human apj apc conjugated antibody, by R&D Systems (1 mentions)
2 protocols using apc mouse anti human cd43
1
Immunophenotyping of Differentiated Cells
2
Immunophenotyping of Progenitor Cells
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Cells were pretreated with Accutase (Invitrogen) for 3 minutes at 37°C and then washed with cold PBS. After 300×g centrifugation, 2×105 cells were resuspended in 100 ml of PBS and incubated with primary antibodies for 30 minutes at 4°C. Then, the cells were washed by using PBS with 2% FBS and 0.2 mM EDTA and used for flow cytometry analysis or sorting. The antibodies were as follows: Human APJ-APC-conjugated Antibody (R&D), PE Mouse Anti-Human CD31 (BD Biosciences), APC Mouse Anti-Human CD34 (BD Biosciences), APC Mouse Anti-Human CD43 (BD Biosciences), Mouse IgG3 APC-conjugated Antibody (R&D), PE Mouse IgG1, κ Isotype Control (BD Biosciences).
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