Hr tem talos f200x

The formation of PT-AuNS was examined using the Ultrospec 6300 pro UV–Vis spectrophotometer (Amersham Biosciences, Buckinghamshire, UK) in the wavelength range of 300–800 nm. The size, zeta potential, and PDI of PT-AuNS were determined using the Zetasizer Nano-ZS90 sample delivery system (Malvern Panalytical, Malvern, UK). The morphology and particle size distribution of PT-AuNS were determined using HR-TEM TALOS F200X (Thermo Scientific, Waltham, MA, USA) at an electron potential of 200 kV. To confirm that PT-AuNS were synthesized via PT extraction, FTIR spectra were obtained using the Spectrum GX spectrometer (Perkin Elmer Inc., Boston, MA, USA) prepared with potassium bromide pellets [47 (link)].

Fungal pellets were harvested and washed with phosphate saline buffer (100 mM, pH 6.8). The obtained fungal cells were fixed using 2.5% v/v glutaraldehyde overnight at 4 °C, and 2% osmium tetroxide was used as a secondary fixative for 2 h. The fixed cells were washed with 0.1 M phosphate buffer. Subsequently, fungal cells were dehydrated in a series of ethanol, cut into ultra thin sections using an ultramicrotome, and fitted on TEM grids for examination [11 (link)]. Samples were visualised using an HR-TEM TALOS F200X (Thermo Fisher Scientific) equipped with a detector of a high-angle annular dark-field (HAADF), Cannon type: Schottky-type field emission (FEG), using the Microanalysis system EDX type (using X-ray energy dispersion) at the Center of Scientific Instrumentation of the University of Granada (Granada, Spain).