Hypoxanthine aminopterin

Citrinin (CIT), Aflatoxin B1 (AFB1), Fumonisin B1 (FB1), Patulin (PAT), Ochratoxin A (OTA), polyethylene glycol (PEG) 1450, hypoxanthine aminopterin and thymidine (HAT) medium, hypoxanthine-thymidine (HT) medium, complete and incomplete Freund adjuvant were purchased from Sigma (USA). The mouse McAb isotyping kit was purchased from Hycult Biotechnology (Netherlands). The SP2/0 myeloma cell line was stored in our lab. The Ridascreen Fast CIT ELISA kit was purchased from the R-Biopharm group (Germany). Goat anti-mouse IgG (H+L) was purchased from BioWorld (USA). Protein-G Sepharose Fast Flow Columns were purchased from GE Healthcare (USA). Chloroauricacid (HAuCl₄) and trisodium citrate were obtained from Shanghai Chemical Reagents (Shanghai, China). The nitrocellulose membranes (Millipore 135, Millipore 180) were purchased from Millipore (USA). Othertypes of nitrocellulose membranes (Sartorius CN 140, PALL Vivid 170), conjugation pads (GL0194, Ahlstrom 8964, Ahlstrom 6613), sample pads (GL-b01, GL-b02) and absorbent pads were purchased from Jieyi Bio. Co. (China).

Endosulfan, toxaphene, chlordane, heptachlor, hypoxanthine aminopterin, bovine serum albumin (BSA), ovalbumin (OVA), carbodiimide (EDC), Freund’s complete adjuvant, Freund’s incomplete adjuvant, chloroauric acid (HAuCl3·4H₂O), trisodium citrate, thymidine medium (HAT) and hypoxanthine-thymidine medium (HT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG, 3,3′,5,5′-tetramethylbenzidine (TMB) and Freund’s complete and incomplete adjuvant were acquired from Sangon Biotech Co., Ltd. (Shanghai, China). Immunogen and coating antigen were prepared in our laboratory. All other reagents were acquired from the National Pharmaceutical Group Chemical Reagent Co., Ltd. (Shanghai, China).
The microplate reader (1201–7044) was obtained from Thermo Fisher Scientific (Shanghai, China) Co., Ltd. (USA). The CT300 CNC strip cutting machine, HM3030 XYZ three-dimensional dispensing platform, ZQ3500 CNC fast chopping machine were obtained from shanghai Kinbio Tech Co., Ltd. (Shanghai, China). The gas chromatography-mass spectrometer (GC-MS) was obtained from Thermo Fischer Scientific (USA).