N-ε-carbobenzoxy-L-lysine (ε-Lys(Z)) and L-phenylalanine (Phe) were purchased from GL Biochem Ltd. (Shanghai, China), and lysine-N-carboxylic acid anhydride (Lys(Z)-NCA) and phenylalanine-N-carboxylic acid anhydride (Phe-NCA) were formed according to a previously reported method [24] . CPT was bought from Dalian Meilun Biology Technology Co., Ltd. (Liaoning, China). Hydrogen bromide solution 33 wt% in acetic acid was purchased from J&K Scientific Ltd (Beijing, China). Paraformaldehyde (PFA) and rhodamine B isothiocyanate (Rho) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Poly(ethylene glycol) (MW 6000) was purchased from JenKem Technology Company (Beijing, China), and α,ω-diformyl poly(ethylene glycol) (OHC-PEG-CHO) was prepared by a previously reported one-pot method [25] (link). Dimethyl sulfoxide (DMSO) and dithiothreitol (DTT) were purchased from Aladdin (Shanghai, China). N, N-dimethylformamide (DMF) was obtained from Damao (Tianjing, China). Pyrene was obtained from Baoman Bio (Shanghai, China). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was obtained from Amresco (Solon, Ohio, USA). An Annexin V-FITC early apoptosis detection kit, DAPI, and a JC-1 MMP assay kit were provided by Beyotime Biotechnology (Nantong, China).
Annexin 5 fitc early apoptosis detection kit
Manufactured by Beyotime
Sourced in China
The Annexin V-FITC early apoptosis detection kit is a laboratory reagent used for the detection and quantification of apoptosis in cell samples. It contains Annexin V conjugated with the fluorescent dye FITC, which binds to phosphatidylserine exposed on the surface of apoptotic cells, allowing their identification through flow cytometry or fluorescence microscopy.
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Lab products found in correlation
Jc 1 mmp assay kit, by Beyotime (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Avantor (1 mentions)
Rhodamine b isothiocyanate, by Merck Group (1 mentions)
Paraformaldehyde pfa, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Ow cytometer, by Beckman Coulter (1 mentions)
Pi rnase staining solution, by Cell Signaling Technology (1 mentions)
2 protocols using annexin 5 fitc early apoptosis detection kit
1
Detailed Synthesis of Functional Polymers
2
Cell Cycle and Apoptosis Analysis
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To examine the effects of the CA on the cell cycle, ow cytometry was employed. The cells were harvested by trypsin digestion after treatment, and xed in 70% cold ethanol (in PBS) overnight at 4°C.
Then PI/RNase staining solution (#4087, cell signaling technology, USA) was added for 20 min (in dark) and the stained cells were tested and counted by ow cytometer. The cells for apoptosis analysis were harvested by trypsin treatment, and stained by Annexin V-FITC Early Apoptosis Detection Kit (C1062M, Beyotime, China) for 20 min in darkness. The stained cells were counted and recorded by ow cytometer (Beckman Coulter, USA).
Then PI/RNase staining solution (#4087, cell signaling technology, USA) was added for 20 min (in dark) and the stained cells were tested and counted by ow cytometer. The cells for apoptosis analysis were harvested by trypsin treatment, and stained by Annexin V-FITC Early Apoptosis Detection Kit (C1062M, Beyotime, China) for 20 min in darkness. The stained cells were counted and recorded by ow cytometer (Beckman Coulter, USA).
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