Acetylcholinesterase (EC 3.1.1.7), α-amylase (EC 3.2.1.1), α-glucosidase (EC 3.2.1.20), lipase (EC 3.1.1.3), and tyrosinase (EC 1.14.18.1) enzymes, as well as murine RAW 264.7 macrophages and B16 A45 melanoma cell lines, were purchased from Sigma-Aldrich (Darmstadt, Germany). Additionally, all reagents (ATChI, ferric chloride, DTNB, Folin–Ciocalteu phenol, L-glutamine, penicillin, potassium persulfate, streptomycin, and TPTZ), standards (acarbose, galantamine, gallic and kojic acids, L-NAME, melanine, and orlistat), radicals (DPPH and ABTS), and solvents (DMSO and methanol and ethanol of analytical grade) used for chemical analyses were supplied by Sigma-Aldrich (St. Louis, MO, USA).
Atchi
Manufactured by Merck Group
Sourced in United States
ATChI is a laboratory instrument used to measure the activity of the enzyme acetylcholinesterase (AChE). AChE is responsible for the breakdown of the neurotransmitter acetylcholine in the body. The ATChI instrument can be used to assess AChE activity in various biological samples, such as blood or tissue, for research or diagnostic purposes.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Acetylcholinesterase, by Merck Group (1 mentions)
Galantamine, by Merck Group (1 mentions)
Potassium persulfate, by Merck Group (1 mentions)
Orlistat, by Merck Group (1 mentions)
Tyrosinase, by Merck Group (1 mentions)
Lipase, by Merck Group (1 mentions)
Ferric chloride, by Merck Group (1 mentions)
L name, by Merck Group (1 mentions)
Folin ciocalteu phenol, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Merck Group (1 mentions)
L glutamine, by Merck Group (1 mentions)
α amylase, by Merck Group (1 mentions)
α glucosidase, by Merck Group (1 mentions)
Acarbose, by Merck Group (1 mentions)
Glycine, by Merck Group (1 mentions)
2 protocols using atchi
1
Standardized Enzyme and Cell Assays
2
Acephate Toxicity Evaluation Protocol
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Acephate (Technical grade, 97.5 %, Meghmani Organics Ltd., Ahmedabad)was received as a gift from the Department of Veterinary Pharmacology and Toxicology, Veterinary College, Anand, AAU, Anand, Gujarat, India, PIN388001. Serum total protein and albumin estimation kits were obtained from Aspen laboratories. DNA molecular weight marker was purchased from MBI Fermentas GERMANY. Boric acid, chloroform, ethanol, glycerol, glycine, HCl, hydrogen peroxide (30 %), isopropanol, isoamyl alcohol, sodium carbonate, methanol were from Merck, Mumbai, INDIA. Ethidium bromide was procured from Hi Media, INDIA. Agarose, DMSO, Con-A, ATChI, PHA-P and other chemicals were purchased from Sigma chemicals, USA. All other reagents used were of analytical grade. Multi-well 24 well plate (35) 3043, 96 well at bottom plate with lid was purchased from Falcon, USA.
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